| http://purl.uniprot.org/citations/31083674 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31083674 | http://www.w3.org/2000/01/rdf-schema#comment | "The genetic programs responsible for pulmonary lymphatic maturation prior to birth are not known. To address this gap in knowledge, we developed a novel cell sorting strategy to collect fetal pulmonary lymphatic endothelial cells (PLECs) for global transcriptional profiling. We identified PLECs based on their unique cell surface immunophenotype (CD31+/Vegfr3+/Lyve1+/Pdpn+) and isolated them from murine lungs during late gestation (E16.5, E17.5, E18.5). Gene expression profiling was performed using whole-genome microarrays, and 1,281 genes were significantly differentially expressed with respect to time (FDR q < 0.05) and grouped into six clusters. Two clusters containing a total of 493 genes strongly upregulated at E18.5 were significantly enriched in genes with functional annotations corresponding to innate immune response, positive regulation of angiogenesis, complement & coagulation cascade, ECM/cell-adhesion, and lipid metabolism. Gene Set Enrichment Analysis identified several pathways coordinately upregulated during late gestation, the strongest of which was the type-I IFN-α/β signaling pathway. Upregulation of canonical interferon target genes was confirmed by qRT-PCR and in situ hybridization in E18.5 PLECs. We also identified transcriptional events consistent with a prenatal PLEC maturation program. This PLEC-specific program included individual genes (Ch25h, Itpkc, Pcdhac2 and S1pr3) as well as a set of chemokines and genes containing an NF-κB binding site in their promoter. Overall, this work reveals transcriptional insights into the genes, signaling pathways and biological processes associated with pulmonary lymphatic maturation in the fetal lung."xsd:string |
| http://purl.uniprot.org/citations/31083674 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0216795"xsd:string |
| http://purl.uniprot.org/citations/31083674 | http://purl.uniprot.org/core/author | "Chen F."xsd:string |
| http://purl.uniprot.org/citations/31083674 | http://purl.uniprot.org/core/author | "Fine A."xsd:string |
| http://purl.uniprot.org/citations/31083674 | http://purl.uniprot.org/core/author | "Gower A.C."xsd:string |
| http://purl.uniprot.org/citations/31083674 | http://purl.uniprot.org/core/author | "Norman T.A. Jr."xsd:string |
| http://purl.uniprot.org/citations/31083674 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/31083674 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
| http://purl.uniprot.org/citations/31083674 | http://purl.uniprot.org/core/pages | "e0216795"xsd:string |
| http://purl.uniprot.org/citations/31083674 | http://purl.uniprot.org/core/title | "Transcriptional landscape of pulmonary lymphatic endothelial cells during fetal gestation."xsd:string |
| http://purl.uniprot.org/citations/31083674 | http://purl.uniprot.org/core/volume | "14"xsd:string |
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