| http://purl.uniprot.org/citations/31093672 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31093672 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31093672 | http://www.w3.org/2000/01/rdf-schema#comment | "CCR4/CAF1 are widely conserved deadenylases in eukaryotes. They form a large complex that includes NOT1 as a scaffold protein and various NOT proteins that are core components of multiple levels of gene expression control. The CCR4-NOT complex also contains several RNA-binding proteins as accessory proteins, which are required for target recognition by CCR4/CAF1 deadenylases. AtCCR4a/b, orthologs of human CCR4 in Arabidopsis, have various physiological effects. AtCCR4 isoforms are likely to have specific target mRNAs related to each physiological effect; however, AtCCR4 does not have RNA-binding capability. Therefore, identifying factors that interact with AtCCR4a/b is indispensable to understand its function as a regulator of gene expression, as well as the target mRNA recognition mechanism. Here, we identified putative components of the AtCCR4-NOT complex using co-immunoprecipitation in combination with mass spectrometry using FLAG-tagged AtCCR4b and subsequent verification with a yeast two-hybrid assay. Interestingly, four of 11 AtCAF1 isoforms interacted with both AtCCR4b and AtNOT1, whereas two isoforms interacted only with AtNOT1 in yeast two-hybrid assays. These results imply that Arabidopsis has multiple CCR4-NOT complexes with various combinations of deadenylases. We also revealed that the RNA-binding protein Arabidopsis Pumilio 5 and 2 interacted with AtCCR4a/b in the cytoplasm with a few foci."xsd:string |
| http://purl.uniprot.org/citations/31093672 | http://purl.org/dc/terms/identifier | "doi:10.1093/pcp/pcz089"xsd:string |
| http://purl.uniprot.org/citations/31093672 | http://purl.org/dc/terms/identifier | "doi:10.1093/pcp/pcz089"xsd:string |
| http://purl.uniprot.org/citations/31093672 | http://purl.uniprot.org/core/author | "Chiba Y."xsd:string |
| http://purl.uniprot.org/citations/31093672 | http://purl.uniprot.org/core/author | "Chiba Y."xsd:string |
| http://purl.uniprot.org/citations/31093672 | http://purl.uniprot.org/core/author | "Suzuki Y."xsd:string |
| http://purl.uniprot.org/citations/31093672 | http://purl.uniprot.org/core/author | "Suzuki Y."xsd:string |
| http://purl.uniprot.org/citations/31093672 | http://purl.uniprot.org/core/author | "Sato T."xsd:string |
| http://purl.uniprot.org/citations/31093672 | http://purl.uniprot.org/core/author | "Sato T."xsd:string |
| http://purl.uniprot.org/citations/31093672 | http://purl.uniprot.org/core/author | "Yamaguchi J."xsd:string |
| http://purl.uniprot.org/citations/31093672 | http://purl.uniprot.org/core/author | "Yamaguchi J."xsd:string |
| http://purl.uniprot.org/citations/31093672 | http://purl.uniprot.org/core/author | "Morita K."xsd:string |
| http://purl.uniprot.org/citations/31093672 | http://purl.uniprot.org/core/author | "Morita K."xsd:string |
| http://purl.uniprot.org/citations/31093672 | http://purl.uniprot.org/core/author | "Yasuda S."xsd:string |
| http://purl.uniprot.org/citations/31093672 | http://purl.uniprot.org/core/author | "Yasuda S."xsd:string |
| http://purl.uniprot.org/citations/31093672 | http://purl.uniprot.org/core/author | "Arae T."xsd:string |
| http://purl.uniprot.org/citations/31093672 | http://purl.uniprot.org/core/author | "Arae T."xsd:string |
| http://purl.uniprot.org/citations/31093672 | http://purl.uniprot.org/core/author | "Imahori R."xsd:string |
| http://purl.uniprot.org/citations/31093672 | http://purl.uniprot.org/core/author | "Imahori R."xsd:string |
| http://purl.uniprot.org/citations/31093672 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/31093672 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/31093672 | http://purl.uniprot.org/core/name | "Plant Cell Physiol."xsd:string |
| http://purl.uniprot.org/citations/31093672 | http://purl.uniprot.org/core/name | "Plant Cell Physiol."xsd:string |