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http://purl.uniprot.org/citations/31103063http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/31103063http://www.w3.org/2000/01/rdf-schema#comment"

Objective

This study aimed to test the influence of homeobox B7 (HoxB7) on the proliferation, invasion, and migration of human trophoblast cells and to reveal the down-regulation of HoxB7 on the transcriptional suppression of Dick Kopf-related protein1 (DKK1) and of Cysteine-rich glycosylated wingless protein 1 (Wnt1)/β-catenin in intrauterine fetal growth retardation (FGR).

Methods

Quantitative measurement of HoxB7, DKK1, Wnt1, and β-catenin was performed in human placentas collected from normal pregnancies and from FGR with quantitative real time PCR (qRT-PCR). Cultured HTR-8/SVneo cells, transfected with a lentiviral plasmid that in-frame expresses human HoxB7 gene, were applied to functional assessment to study the biological impact of HoxB7 gene on DKK1, Wnt1, and β-catenin. Counting Kit-8, Transwell invasion assays, and flow cytometry were applied for the functional measurements.

Results

The expression of HoxB7 was significantly increased, and of DKK1, Wnt1, and β-catenin was decreased, in FGR placenta tissues and in HTR-8/SVneo cells. Function studies revealed that overexpression of HoxB7 inhibited proliferation, migration, and invasion in HTR-8/SVneo cells. DKK1, Wnt1, and β-catenin were down-regulated in HTR-8/SVneo cells, inversely correlated with HoxB7 expression. Overexpression of HoxB7 showed a suppressive effect on proliferation, migration, and invasion in the HTR-8/SVneo cells.

Conclusions

Our results indicate that HoxB7 inhibited human trophoblast cell differentiation by down-regulating DKK1 expression and that it may affect transcription of Wnt1/β-catenin. The activation of HoxB7 might suppress the cell differentiation in HTR-8/SVneo cell cultures. The Wnt/β-catenin signaling pathway may play a significant role in the pathogenesis of FGR by regulating the invasion and proliferation of trophoblasts."xsd:string
http://purl.uniprot.org/citations/31103063http://purl.org/dc/terms/identifier"doi:10.1016/j.placenta.2019.03.001"xsd:string
http://purl.uniprot.org/citations/31103063http://purl.uniprot.org/core/author"Chen Z."xsd:string
http://purl.uniprot.org/citations/31103063http://purl.uniprot.org/core/author"Chen D."xsd:string
http://purl.uniprot.org/citations/31103063http://purl.uniprot.org/core/author"Huang L."xsd:string
http://purl.uniprot.org/citations/31103063http://purl.uniprot.org/core/author"Gu Y."xsd:string
http://purl.uniprot.org/citations/31103063http://purl.uniprot.org/core/author"Hu L."xsd:string
http://purl.uniprot.org/citations/31103063http://purl.uniprot.org/core/author"Ying H."xsd:string
http://purl.uniprot.org/citations/31103063http://purl.uniprot.org/core/author"Zhong N."xsd:string
http://purl.uniprot.org/citations/31103063http://purl.uniprot.org/core/author"Zhu Y.L."xsd:string
http://purl.uniprot.org/citations/31103063http://purl.uniprot.org/core/date"2019"xsd:gYear
http://purl.uniprot.org/citations/31103063http://purl.uniprot.org/core/name"Placenta"xsd:string
http://purl.uniprot.org/citations/31103063http://purl.uniprot.org/core/pages"27-35"xsd:string
http://purl.uniprot.org/citations/31103063http://purl.uniprot.org/core/title"Down-regulation of DKK1 and Wnt1/beta-catenin pathway by increased homeobox B7 resulted in cell differentiation suppression of intrauterine fetal growth retardation in human placenta."xsd:string
http://purl.uniprot.org/citations/31103063http://purl.uniprot.org/core/volume"80"xsd:string
http://purl.uniprot.org/citations/31103063http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/31103063
http://purl.uniprot.org/citations/31103063http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/31103063
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http://purl.uniprot.org/uniprot/#_P09629-mappedCitation-31103063http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/31103063
http://purl.uniprot.org/uniprot/#_O94907-mappedCitation-31103063http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/31103063
http://purl.uniprot.org/uniprot/#_Q9HB12-mappedCitation-31103063http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/31103063
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http://purl.uniprot.org/uniprot/P09629http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/31103063
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