| http://purl.uniprot.org/citations/31131408 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31131408 | http://www.w3.org/2000/01/rdf-schema#comment | "Bacterial RNA polymerase (RNAP) forms distinct holoenzymes with extra-cytoplasmic function (ECF) σ factors to initiate specific gene expression programs. In this study, we report a cryo-EM structure at 4.0 Å of Escherichia coli transcription initiation complex comprising σE-the most-studied bacterial ECF σ factor (Ec σE-RPo), and a crystal structure at 3.1 Å of Mycobacterium tuberculosis transcription initiation complex with a chimeric σH/E (Mtb σH/E-RPo). The structure of Ec σE-RPo reveals key interactions essential for assembly of E. coli σE-RNAP holoenzyme and for promoter recognition and unwinding by E. coli σE. Moreover, both structures show that the non-conserved linkers (σ2/σ4 linker) of the two ECF σ factors are inserted into the active-center cleft and exit through the RNA-exit channel. We performed secondary-structure prediction of 27,670 ECF σ factors and find that their non-conserved linkers probably reach into and exit from RNAP active-center cleft in a similar manner. Further biochemical results suggest that such σ2/σ4 linker plays an important role in RPo formation, abortive production and promoter escape during ECF σ factors-mediated transcription initiation."xsd:string |
| http://purl.uniprot.org/citations/31131408 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gkz470"xsd:string |
| http://purl.uniprot.org/citations/31131408 | http://purl.uniprot.org/core/author | "Feng Y."xsd:string |
| http://purl.uniprot.org/citations/31131408 | http://purl.uniprot.org/core/author | "Li L."xsd:string |
| http://purl.uniprot.org/citations/31131408 | http://purl.uniprot.org/core/author | "Shen L."xsd:string |
| http://purl.uniprot.org/citations/31131408 | http://purl.uniprot.org/core/author | "Shi J."xsd:string |
| http://purl.uniprot.org/citations/31131408 | http://purl.uniprot.org/core/author | "Zhang Y."xsd:string |
| http://purl.uniprot.org/citations/31131408 | http://purl.uniprot.org/core/author | "Wang S."xsd:string |
| http://purl.uniprot.org/citations/31131408 | http://purl.uniprot.org/core/author | "Fang C."xsd:string |
| http://purl.uniprot.org/citations/31131408 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/31131408 | http://purl.uniprot.org/core/name | "Nucleic Acids Res"xsd:string |
| http://purl.uniprot.org/citations/31131408 | http://purl.uniprot.org/core/pages | "7094-7104"xsd:string |
| http://purl.uniprot.org/citations/31131408 | http://purl.uniprot.org/core/title | "Structures and mechanism of transcription initiation by bacterial ECF factors."xsd:string |
| http://purl.uniprot.org/citations/31131408 | http://purl.uniprot.org/core/volume | "47"xsd:string |
| http://purl.uniprot.org/citations/31131408 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/31131408 |
| http://purl.uniprot.org/citations/31131408 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/31131408 |
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