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http://purl.uniprot.org/citations/31138033http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/31138033http://www.w3.org/2000/01/rdf-schema#comment"

Purpose

Glioma is identified as a broad category of brain and spinal cord tumors. MiR-32 is important in regulating the genesis of different cancers; however, the underlying mechanisms of miR-32 in glioma still largely unknown. This study aimed to elucidate pathobiological functions of miR-32 in glioma and verify its effect on the regulation of enhancer of zeste homolog 2.

Methods

The expression of miR-32 and enhancer of zeste homolog 2 was detected by quantitative real-time polymerase chain reaction and Western blot in glioma tissues and cells. Cell Counting Kit-8 (CCK-8) assay was used to examine the effects of miR-32 on human glioma cells proliferation. Transwell assay was used to examine cell metastasis, respectively. Two bioinformatics analysis software and luciferase reporter assay were chosen to confirm targeting association between miR-32 and enhancer of zeste homolog 2.

Results

MiR-32 was downregulated in glioma tissues and cells. Furthermore, enhancer of zeste homolog 2 expression was upregulated and negatively correlated with miR-32 in clinical tissues. Ectopic expression of miR-32 inhibited glioma cell proliferation, migration, and invasion. Enhancer of zeste homolog 2 was identified as direct target gene of miR-32 in glioma. Overexpression of enhancer of zeste homolog 2 ablated the inhibitory effects of miR-32.

Conclusion

In summary, our finding suggests that miR-32 acts an important role in inhibiting glioma cell proliferation and metastasis and suppresses the expression of ABCC4 by directly targeting its 3'-untranslated region. The miR-32/enhancer of zeste homolog 2 axis may provide new insights to the treatment for glioma."xsd:string
http://purl.uniprot.org/citations/31138033http://purl.org/dc/terms/identifier"doi:10.1177/1533033819854132"xsd:string
http://purl.uniprot.org/citations/31138033http://purl.uniprot.org/core/author"Chen F."xsd:string
http://purl.uniprot.org/citations/31138033http://purl.uniprot.org/core/author"Chen C."xsd:string
http://purl.uniprot.org/citations/31138033http://purl.uniprot.org/core/author"Chen H."xsd:string
http://purl.uniprot.org/citations/31138033http://purl.uniprot.org/core/author"Zhang Y."xsd:string
http://purl.uniprot.org/citations/31138033http://purl.uniprot.org/core/author"Wang J."xsd:string
http://purl.uniprot.org/citations/31138033http://purl.uniprot.org/core/author"Zheng W."xsd:string
http://purl.uniprot.org/citations/31138033http://purl.uniprot.org/core/author"Wang W."xsd:string
http://purl.uniprot.org/citations/31138033http://purl.uniprot.org/core/author"Zhu C."xsd:string
http://purl.uniprot.org/citations/31138033http://purl.uniprot.org/core/author"Gong J."xsd:string
http://purl.uniprot.org/citations/31138033http://purl.uniprot.org/core/author"An W."xsd:string
http://purl.uniprot.org/citations/31138033http://purl.uniprot.org/core/date"2019"xsd:gYear
http://purl.uniprot.org/citations/31138033http://purl.uniprot.org/core/name"Technol Cancer Res Treat"xsd:string
http://purl.uniprot.org/citations/31138033http://purl.uniprot.org/core/pages"1533033819854132"xsd:string
http://purl.uniprot.org/citations/31138033http://purl.uniprot.org/core/title"MiR-32 Inhibits Proliferation and Metastasis by Targeting EZH2 in Glioma."xsd:string
http://purl.uniprot.org/citations/31138033http://purl.uniprot.org/core/volume"18"xsd:string
http://purl.uniprot.org/citations/31138033http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/31138033
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