| http://purl.uniprot.org/citations/31140975 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31140975 | http://www.w3.org/2000/01/rdf-schema#comment | "Fluorescent transcriptional reporters are widely used as signaling reporters and biomarkers to monitor pathway activities and determine cell type identities. However, a large amount of dynamic information is lost due to the long half-life of the fluorescent proteins. To better detect dynamics, fluorescent transcriptional reporters can be destabilized to shorten their half-lives. However, applications of this approach in vivo are limited due to significant reduction of signal intensities. To overcome this limitation, we enhanced translation of a destabilized fluorescent protein and demonstrate the advantages of this approach by characterizing spatio-temporal changes of transcriptional activities in Drosophila. In addition, by combining a fast-folding destabilized fluorescent protein and a slow-folding long-lived fluorescent protein, we generated a dual-color transcriptional timer that provides spatio-temporal information about signaling pathway activities. Finally, we demonstrate the use of this transcriptional timer to identify new genes with dynamic expression patterns."xsd:string |
| http://purl.uniprot.org/citations/31140975 | http://purl.org/dc/terms/identifier | "doi:10.7554/elife.46181"xsd:string |
| http://purl.uniprot.org/citations/31140975 | http://purl.uniprot.org/core/author | "Huang J."xsd:string |
| http://purl.uniprot.org/citations/31140975 | http://purl.uniprot.org/core/author | "Perrimon N."xsd:string |
| http://purl.uniprot.org/citations/31140975 | http://purl.uniprot.org/core/author | "He L."xsd:string |
| http://purl.uniprot.org/citations/31140975 | http://purl.uniprot.org/core/author | "Binari R."xsd:string |
| http://purl.uniprot.org/citations/31140975 | http://purl.uniprot.org/core/author | "Falo-Sanjuan J."xsd:string |
| http://purl.uniprot.org/citations/31140975 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/31140975 | http://purl.uniprot.org/core/name | "Elife"xsd:string |
| http://purl.uniprot.org/citations/31140975 | http://purl.uniprot.org/core/pages | "e46181"xsd:string |
| http://purl.uniprot.org/citations/31140975 | http://purl.uniprot.org/core/title | "In vivo study of gene expression with an enhanced dual-color fluorescent transcriptional timer."xsd:string |
| http://purl.uniprot.org/citations/31140975 | http://purl.uniprot.org/core/volume | "8"xsd:string |
| http://purl.uniprot.org/citations/31140975 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/31140975 |
| http://purl.uniprot.org/citations/31140975 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/31140975 |
| http://purl.uniprot.org/uniprot/#_A0A0B4LF57-mappedCitation-31140975 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31140975 |
| http://purl.uniprot.org/uniprot/#_A0A0B4K6K9-mappedCitation-31140975 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31140975 |
| http://purl.uniprot.org/uniprot/#_A0A0B4K6Q6-mappedCitation-31140975 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31140975 |
| http://purl.uniprot.org/uniprot/#_A0A0B4K7Y7-mappedCitation-31140975 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31140975 |
| http://purl.uniprot.org/uniprot/#_A0A0B4JDG1-mappedCitation-31140975 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31140975 |
| http://purl.uniprot.org/uniprot/#_A0A0B4K630-mappedCitation-31140975 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31140975 |
| http://purl.uniprot.org/uniprot/#_A0A0B4JD39-mappedCitation-31140975 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31140975 |
| http://purl.uniprot.org/uniprot/#_A4V3W8-mappedCitation-31140975 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31140975 |
| http://purl.uniprot.org/uniprot/#_C7LAF5-mappedCitation-31140975 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31140975 |
| http://purl.uniprot.org/uniprot/#_A4V1F9-mappedCitation-31140975 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31140975 |