| http://purl.uniprot.org/citations/3114239 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/3114239 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/3114239 | http://www.w3.org/2000/01/rdf-schema#comment | "A chromosomal gene of Streptococcus pneumoniae carrying a spontaneous mutation to sulfonamide resistance was identified. Comparison of its DNA sequence with the wild-type sequence showed that the mutation, sul-d, consisted of an insert of 6 base pairs, a repeat of an adjacent 6-base-pair segment. The gene encoded a 34-kilodalton polypeptide, SulA, which as a dimer or trimer constituted the enzyme dihydropteroate synthase. This was shown by enzyme activity measurements, expression in minicells of Bacillus subtilis, and the amino-terminal sequence of the polypeptide product. Subcloning of the gene in an Escherichia coli expression vector allowed purification of the enzyme to 80% homogeneity in a single step and at high yield. Although a deleted plasmid, pLS83, produced the mutant dihydropteroate synthase, it did not confer sulfonamide resistance in vivo. It is suggested that the SulA polypeptide is also a component of an enzyme that acts in another step of folate biosynthesis and that this step is inhibited in vivo by either free or conjugated sulfonamides."xsd:string |
| http://purl.uniprot.org/citations/3114239 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.169.9.4320-4326.1987"xsd:string |
| http://purl.uniprot.org/citations/3114239 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.169.9.4320-4326.1987"xsd:string |
| http://purl.uniprot.org/citations/3114239 | http://purl.uniprot.org/core/author | "Lopez P."xsd:string |
| http://purl.uniprot.org/citations/3114239 | http://purl.uniprot.org/core/author | "Lopez P."xsd:string |
| http://purl.uniprot.org/citations/3114239 | http://purl.uniprot.org/core/author | "Greenberg B."xsd:string |
| http://purl.uniprot.org/citations/3114239 | http://purl.uniprot.org/core/author | "Greenberg B."xsd:string |
| http://purl.uniprot.org/citations/3114239 | http://purl.uniprot.org/core/author | "Lacks S.A."xsd:string |
| http://purl.uniprot.org/citations/3114239 | http://purl.uniprot.org/core/author | "Lacks S.A."xsd:string |
| http://purl.uniprot.org/citations/3114239 | http://purl.uniprot.org/core/author | "Espinosa M."xsd:string |
| http://purl.uniprot.org/citations/3114239 | http://purl.uniprot.org/core/author | "Espinosa M."xsd:string |
| http://purl.uniprot.org/citations/3114239 | http://purl.uniprot.org/core/date | "1987"xsd:gYear |
| http://purl.uniprot.org/citations/3114239 | http://purl.uniprot.org/core/date | "1987"xsd:gYear |
| http://purl.uniprot.org/citations/3114239 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/3114239 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/3114239 | http://purl.uniprot.org/core/pages | "4320-4326"xsd:string |
| http://purl.uniprot.org/citations/3114239 | http://purl.uniprot.org/core/pages | "4320-4326"xsd:string |
| http://purl.uniprot.org/citations/3114239 | http://purl.uniprot.org/core/title | "Sulfonamide resistance in Streptococcus pneumoniae: DNA sequence of the gene encoding dihydropteroate synthase and characterization of the enzyme."xsd:string |
| http://purl.uniprot.org/citations/3114239 | http://purl.uniprot.org/core/title | "Sulfonamide resistance in Streptococcus pneumoniae: DNA sequence of the gene encoding dihydropteroate synthase and characterization of the enzyme."xsd:string |
| http://purl.uniprot.org/citations/3114239 | http://purl.uniprot.org/core/volume | "169"xsd:string |
| http://purl.uniprot.org/citations/3114239 | http://purl.uniprot.org/core/volume | "169"xsd:string |
| http://purl.uniprot.org/citations/3114239 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/3114239 |
| http://purl.uniprot.org/citations/3114239 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/3114239 |