| http://purl.uniprot.org/citations/31155309 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31155309 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31155309 | http://www.w3.org/2000/01/rdf-schema#comment | "Ric-8A is a 530-amino acid cytoplasmic molecular chaperone and guanine nucleotide exchange factor (GEF) for i, q, and 12/13 classes of heterortrimeric G protein alpha subunits (Gα). We report the 2.2-Å crystal structure of the Ric-8A Gα-binding domain with GEF activity, residues 1-452, and is phosphorylated at Ser435 and Thr440. Residues 1-429 adopt a superhelical fold comprised of Armadillo (ARM) and HEAT repeats, and the C terminus is disordered. One of the phosphorylated residues potentially binds to a basic cluster in an ARM motif. Amino acid sequence conservation and published hydrogen-deuterium exchange data indicate repeats 3 through 6 to be a putative Gα-binding surface. Normal mode modeling of small-angle X-ray scattering data indicates that phosphorylation induces relative rotation between repeats 1-4, 5-6, and 7-9. 2D 1H-15N-TROSY spectra of [2H,15N]-labeled Gαi1 in the presence of R452 reveals chemical shift perturbations of the C terminus and Gαi1 residues involved in nucleotide binding."xsd:string |
| http://purl.uniprot.org/citations/31155309 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.str.2019.04.013"xsd:string |
| http://purl.uniprot.org/citations/31155309 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.str.2019.04.013"xsd:string |
| http://purl.uniprot.org/citations/31155309 | http://purl.uniprot.org/core/author | "Yu W."xsd:string |
| http://purl.uniprot.org/citations/31155309 | http://purl.uniprot.org/core/author | "Yu W."xsd:string |
| http://purl.uniprot.org/citations/31155309 | http://purl.uniprot.org/core/author | "Steiner A."xsd:string |
| http://purl.uniprot.org/citations/31155309 | http://purl.uniprot.org/core/author | "Steiner A."xsd:string |
| http://purl.uniprot.org/citations/31155309 | http://purl.uniprot.org/core/author | "Doukov T.I."xsd:string |
| http://purl.uniprot.org/citations/31155309 | http://purl.uniprot.org/core/author | "Doukov T.I."xsd:string |
| http://purl.uniprot.org/citations/31155309 | http://purl.uniprot.org/core/author | "Hagn F."xsd:string |
| http://purl.uniprot.org/citations/31155309 | http://purl.uniprot.org/core/author | "Hagn F."xsd:string |
| http://purl.uniprot.org/citations/31155309 | http://purl.uniprot.org/core/author | "Zeng B."xsd:string |
| http://purl.uniprot.org/citations/31155309 | http://purl.uniprot.org/core/author | "Zeng B."xsd:string |
| http://purl.uniprot.org/citations/31155309 | http://purl.uniprot.org/core/author | "Mou T.C."xsd:string |
| http://purl.uniprot.org/citations/31155309 | http://purl.uniprot.org/core/author | "Mou T.C."xsd:string |
| http://purl.uniprot.org/citations/31155309 | http://purl.uniprot.org/core/author | "Sprang S.R."xsd:string |
| http://purl.uniprot.org/citations/31155309 | http://purl.uniprot.org/core/author | "Sprang S.R."xsd:string |
| http://purl.uniprot.org/citations/31155309 | http://purl.uniprot.org/core/author | "Tall G.G."xsd:string |
| http://purl.uniprot.org/citations/31155309 | http://purl.uniprot.org/core/author | "Tall G.G."xsd:string |
| http://purl.uniprot.org/citations/31155309 | http://purl.uniprot.org/core/author | "Papasergi-Scott M."xsd:string |
| http://purl.uniprot.org/citations/31155309 | http://purl.uniprot.org/core/author | "Papasergi-Scott M."xsd:string |
| http://purl.uniprot.org/citations/31155309 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/31155309 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |