| http://purl.uniprot.org/citations/31160578 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31160578 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31160578 | http://www.w3.org/2000/01/rdf-schema#comment | "DNA replication initiation is a two-step process. During the G1-phase of the cell cycle, the ORC complex, CDC6, CDT1, and MCM2-7 assemble at replication origins, forming pre-replicative complexes (pre-RCs). In S-phase, kinase activities allow fork establishment through (CDC45/MCM2-7/GINS) CMG-complex formation. However, only a subset of all potential origins becomes activated, through a poorly understood selection mechanism. Here we analyse the pre-RC proteomic interactome in human cells and find C13ORF7/RNF219 (hereafter called OBI1, for ORC-ubiquitin-ligase-1) associated with the ORC complex. OBI1 silencing result in defective origin firing, as shown by reduced CMG formation, without affecting pre-RC establishment. OBI1 catalyses the multi-mono-ubiquitylation of a subset of chromatin-bound ORC3 and ORC5 during S-phase. Importantly, expression of non-ubiquitylable ORC3/5 mutants impairs origin firing, demonstrating their relevance as OBI1 substrates for origin firing. Our results identify a ubiquitin signalling pathway involved in origin activation and provide a candidate protein for selecting the origins to be fired."xsd:string |
| http://purl.uniprot.org/citations/31160578 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41467-019-10321-x"xsd:string |
| http://purl.uniprot.org/citations/31160578 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41467-019-10321-x"xsd:string |
| http://purl.uniprot.org/citations/31160578 | http://purl.uniprot.org/core/author | "Bocquet S."xsd:string |
| http://purl.uniprot.org/citations/31160578 | http://purl.uniprot.org/core/author | "Bocquet S."xsd:string |
| http://purl.uniprot.org/citations/31160578 | http://purl.uniprot.org/core/author | "Coulombe P."xsd:string |
| http://purl.uniprot.org/citations/31160578 | http://purl.uniprot.org/core/author | "Coulombe P."xsd:string |
| http://purl.uniprot.org/citations/31160578 | http://purl.uniprot.org/core/author | "Mechali M."xsd:string |
| http://purl.uniprot.org/citations/31160578 | http://purl.uniprot.org/core/author | "Mechali M."xsd:string |
| http://purl.uniprot.org/citations/31160578 | http://purl.uniprot.org/core/author | "Delamarre A."xsd:string |
| http://purl.uniprot.org/citations/31160578 | http://purl.uniprot.org/core/author | "Delamarre A."xsd:string |
| http://purl.uniprot.org/citations/31160578 | http://purl.uniprot.org/core/author | "Peiffer I."xsd:string |
| http://purl.uniprot.org/citations/31160578 | http://purl.uniprot.org/core/author | "Peiffer I."xsd:string |
| http://purl.uniprot.org/citations/31160578 | http://purl.uniprot.org/core/author | "Nassar J."xsd:string |
| http://purl.uniprot.org/citations/31160578 | http://purl.uniprot.org/core/author | "Nassar J."xsd:string |
| http://purl.uniprot.org/citations/31160578 | http://purl.uniprot.org/core/author | "Stanojcic S."xsd:string |
| http://purl.uniprot.org/citations/31160578 | http://purl.uniprot.org/core/author | "Stanojcic S."xsd:string |
| http://purl.uniprot.org/citations/31160578 | http://purl.uniprot.org/core/author | "Sterkers Y."xsd:string |
| http://purl.uniprot.org/citations/31160578 | http://purl.uniprot.org/core/author | "Sterkers Y."xsd:string |
| http://purl.uniprot.org/citations/31160578 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/31160578 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/31160578 | http://purl.uniprot.org/core/name | "Nat. Commun."xsd:string |
| http://purl.uniprot.org/citations/31160578 | http://purl.uniprot.org/core/name | "Nat. Commun."xsd:string |