| http://purl.uniprot.org/citations/31208963 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31208963 | http://www.w3.org/2000/01/rdf-schema#comment | "Gametogenesis is dependent on intercellular communication facilitated by stable intercellular bridges connecting developing germ cells. During Drosophila oogenesis, intercellular bridges (referred to as ring canals; RCs) have a dynamic actin cytoskeleton that drives their expansion to a diameter of 10 μm. Although multiple proteins have been identified as components of RCs, we lack a basic understanding of how RC proteins interact together to form and regulate the RC cytoskeleton. Thus, here, we optimized a procedure for proximity-dependent biotinylation in live tissue using the APEX enzyme to interrogate the RC interactome. APEX was fused to four different RC components (RC-APEX baits) and 55 unique high-confidence prey were identified. The RC-APEX baits produced almost entirely distinct interactomes that included both known RC proteins and uncharacterized proteins. A proximity ligation assay was used to validate close-proximity interactions between the RC-APEX baits and their respective prey. Furthermore, an RNA interference screen revealed functional roles for several high-confidence prey genes in RC biology. These findings highlight the utility of enzyme-catalyzed proximity labeling for protein interactome analysis in live tissue and expand our understanding of RC biology."xsd:string |
| http://purl.uniprot.org/citations/31208963 | http://purl.org/dc/terms/identifier | "doi:10.1242/dev.176644"xsd:string |
| http://purl.uniprot.org/citations/31208963 | http://purl.uniprot.org/core/author | "Cooley L."xsd:string |
| http://purl.uniprot.org/citations/31208963 | http://purl.uniprot.org/core/author | "Kaufman R.S."xsd:string |
| http://purl.uniprot.org/citations/31208963 | http://purl.uniprot.org/core/author | "Mannix K.M."xsd:string |
| http://purl.uniprot.org/citations/31208963 | http://purl.uniprot.org/core/author | "Starble R.M."xsd:string |
| http://purl.uniprot.org/citations/31208963 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/31208963 | http://purl.uniprot.org/core/name | "Development"xsd:string |
| http://purl.uniprot.org/citations/31208963 | http://purl.uniprot.org/core/pages | "dev176644"xsd:string |
| http://purl.uniprot.org/citations/31208963 | http://purl.uniprot.org/core/title | "Proximity labeling reveals novel interactomes in live Drosophila tissue."xsd:string |
| http://purl.uniprot.org/citations/31208963 | http://purl.uniprot.org/core/volume | "146"xsd:string |
| http://purl.uniprot.org/citations/31208963 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/31208963 |
| http://purl.uniprot.org/citations/31208963 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/31208963 |
| http://purl.uniprot.org/uniprot/#_A0A0B4KHN1-mappedCitation-31208963 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31208963 |
| http://purl.uniprot.org/uniprot/#_A0A0B4KFC5-mappedCitation-31208963 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31208963 |
| http://purl.uniprot.org/uniprot/#_A0A0B4LF29-mappedCitation-31208963 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31208963 |
| http://purl.uniprot.org/uniprot/#_A0A0B4LFC7-mappedCitation-31208963 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31208963 |
| http://purl.uniprot.org/uniprot/#_A0A0B4K6T1-mappedCitation-31208963 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31208963 |
| http://purl.uniprot.org/uniprot/#_A0A0B4K6Y7-mappedCitation-31208963 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31208963 |
| http://purl.uniprot.org/uniprot/#_A0A0B4K6Z1-mappedCitation-31208963 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31208963 |
| http://purl.uniprot.org/uniprot/#_E1JH02-mappedCitation-31208963 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31208963 |
| http://purl.uniprot.org/uniprot/#_E1JHA4-mappedCitation-31208963 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31208963 |
| http://purl.uniprot.org/uniprot/#_P10383-mappedCitation-31208963 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31208963 |
| http://purl.uniprot.org/uniprot/#_A0A0B4K7U5-mappedCitation-31208963 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31208963 |