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http://purl.uniprot.org/citations/31221220http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/31221220http://www.w3.org/2000/01/rdf-schema#comment"

Background

In order to prepare the genome for gametogenesis, primordial germ cells (PGCs) undergo extensive epigenetic reprogramming during migration toward the gonads in mammalian embryos. This includes changes on a genome-wide scale and additionally in females the remodeling of the inactive X-chromosome to enable X-chromosome reactivation (XCR). However, if global remodeling and X-chromosomal remodeling are related, how they occur in PGCs in vivo in relation to their migration progress and which factors are important are unknown.

Results

Here we identify the germ cell determinant PR-domain containing protein 14 (PRDM14) as the first known factor that is instrumental for both global reprogramming and X-chromosomal reprogramming in migrating mouse PGCs. We find that global upregulation of the repressive histone H3 lysine 27 trimethylation (H3K27me3) mark is PRDM14 dosage dependent in PGCs of both sexes. When focusing on XCR, we observed that PRDM14 is required for removal of H3K27me3 from the inactive X-chromosome, which, in contrast to global upregulation, takes place progressively along the PGC migration path. Furthermore, we show that global and X-chromosomal reprogramming of H3K27me3 are functionally separable, despite their common regulation by PRDM14.

Conclusions

In summary, here we provide new insight and spatiotemporal resolution to the progression and regulation of epigenome remodeling along mouse PGC migration in vivo and link epigenetic reprogramming to its developmental context."xsd:string
http://purl.uniprot.org/citations/31221220http://purl.org/dc/terms/identifier"doi:10.1186/s13072-019-0284-7"xsd:string
http://purl.uniprot.org/citations/31221220http://purl.uniprot.org/core/author"Payer B."xsd:string
http://purl.uniprot.org/citations/31221220http://purl.uniprot.org/core/author"Guirola M."xsd:string
http://purl.uniprot.org/citations/31221220http://purl.uniprot.org/core/author"Mallol A."xsd:string
http://purl.uniprot.org/citations/31221220http://purl.uniprot.org/core/date"2019"xsd:gYear
http://purl.uniprot.org/citations/31221220http://purl.uniprot.org/core/name"Epigenetics Chromatin"xsd:string
http://purl.uniprot.org/citations/31221220http://purl.uniprot.org/core/pages"38"xsd:string
http://purl.uniprot.org/citations/31221220http://purl.uniprot.org/core/title"PRDM14 controls X-chromosomal and global epigenetic reprogramming of H3K27me3 in migrating mouse primordial germ cells."xsd:string
http://purl.uniprot.org/citations/31221220http://purl.uniprot.org/core/volume"12"xsd:string
http://purl.uniprot.org/citations/31221220http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/31221220
http://purl.uniprot.org/citations/31221220http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/31221220
http://purl.uniprot.org/uniprot/#_A0A023UED4-mappedCitation-31221220http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/31221220
http://purl.uniprot.org/uniprot/#_E9Q3T6-mappedCitation-31221220http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/31221220
http://purl.uniprot.org/uniprot/A0A023UED4http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/31221220
http://purl.uniprot.org/uniprot/E9Q3T6http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/31221220