| http://purl.uniprot.org/citations/31237474 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31237474 | http://www.w3.org/2000/01/rdf-schema#comment | "Cytoskeletal rearrangement and acute cytotoxicity occur in Vibrio vulnificus-infected host cells. RtxA1 toxin, a multifunctional autoprocessing repeats-in-toxin (MARTX), is essential for the pathogenesis of V. vulnificus and the programmed necrotic cell death. In this study, HeLa cells expressing RtxA1 amino acids 1491-1971 fused to GFP were observed to be rounded. Through yeast two-hybrid screening and subsequent immunoprecipitation validation assays, we confirmed the specific binding of a RtxA11491-1971 fragment with host-cell filamin A, an actin cross-linking scaffold protein. Downregulation of filamin A expression decreased the cytotoxicity of RtxA1 toward host cells. Furthermore, the phosphorylation of JNK and p38 MAPKs was induced by the RtxA1-filamin A interaction during the toxin-mediated cell death. However, the phosphorylation of these MAPKs was not observed during the RtxA1 intoxication of filamin A-deficient M2 cells. In addition, the depletion of pak1, which appeared to be activated by the RtxA1-filamin A interaction, inhibited RtxA1-induced phosphorylation of JNK and p38, and the cells treated with a pak1 inhibitor exhibited decreased RtxA1-mediated cytoskeletal rearrangement and cytotoxicity. Thus, the binding of filamin A by the RtxA11491-1971 domain appears to be a requisite to pak1-mediated MAPK activation, which contributes to the cytoskeletal reorganization and host cell death."xsd:string |
| http://purl.uniprot.org/citations/31237474 | http://purl.org/dc/terms/identifier | "doi:10.1080/22221751.2019.1632153"xsd:string |
| http://purl.uniprot.org/citations/31237474 | http://purl.uniprot.org/core/author | "Jeong K."xsd:string |
| http://purl.uniprot.org/citations/31237474 | http://purl.uniprot.org/core/author | "Rhee J.H."xsd:string |
| http://purl.uniprot.org/citations/31237474 | http://purl.uniprot.org/core/author | "Im Y.J."xsd:string |
| http://purl.uniprot.org/citations/31237474 | http://purl.uniprot.org/core/author | "Kim Y.R."xsd:string |
| http://purl.uniprot.org/citations/31237474 | http://purl.uniprot.org/core/author | "Guo R.H."xsd:string |
| http://purl.uniprot.org/citations/31237474 | http://purl.uniprot.org/core/author | "Shin S.I."xsd:string |
| http://purl.uniprot.org/citations/31237474 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/31237474 | http://purl.uniprot.org/core/name | "Emerg Microbes Infect"xsd:string |
| http://purl.uniprot.org/citations/31237474 | http://purl.uniprot.org/core/pages | "934-945"xsd:string |
| http://purl.uniprot.org/citations/31237474 | http://purl.uniprot.org/core/title | "Vibrio vulnificus RtxA1 cytotoxin targets filamin A to regulate PAK1- and MAPK-dependent cytoskeleton reorganization and cell death."xsd:string |
| http://purl.uniprot.org/citations/31237474 | http://purl.uniprot.org/core/volume | "8"xsd:string |
| http://purl.uniprot.org/citations/31237474 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/31237474 |
| http://purl.uniprot.org/citations/31237474 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/31237474 |
| http://purl.uniprot.org/uniprot/#_A0A075TSG1-mappedCitation-31237474 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31237474 |
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| http://purl.uniprot.org/uniprot/#_B4DTD5-mappedCitation-31237474 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31237474 |
| http://purl.uniprot.org/uniprot/#_B4E2F9-mappedCitation-31237474 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31237474 |
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