http://purl.uniprot.org/citations/31280432 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/31280432 | http://www.w3.org/2000/01/rdf-schema#comment | "IntroductionInflammation is a key aspect of glioblastoma multiforme (GBM) although it remains unclear how it contributes to GBM pathogenesis. Inflammasomes are intracellular multi-protein complexes that are involved in innate immunity and are activated by cellular stress, principally in macrophages. This study examined the expression of inflammasome-associated genes in GBM, particularly absent in melanoma 2 (AIM2).MethodsTissue samples from surgically-resected GBM tumors (nā=ā10) were compared to resected brain specimens from patients with epilepsy (age- and sex-matched Other Disease Controls (ODC, n=5)) by qRT-PCR, western blotting and immunofluorescence. Gene expression studies in human astrocytoma U251 cells were performed and the effects of deleting the absent in melanoma 2 (AIM2) gene using the CRISPR-Cas9 system were analyzed.ResultsGBM tissues showed significantly elevated expression of multiple immune (CD3E, CD163, CD68, MX1, ARG1) and inflammasome (AIM2, NLRP1, IL18, CASP1, and IL-33) genes compared to ODC tissues, without induction of IL1B, IFNG or TNFA. An insert-containing AIM2 variant transcript was highly expressed in GBM tissues and in U251 cells. AIM2 immunoreactivity was concentrated in the tumor core in the absence of PCNA immunodetection and showed a predominant 52 kDa immunoreactive band on western blot. Deletion of AIM2 resulted in significantly enhanced proliferation of U251 cells, which also displayed increased resistance to temozolomide treatment.ConclusionsGBM tumors express a distinct profile of inflammasome-associated genes in a tumor-specific manner. AIM2 expression in tumor cells suppressed cell proliferation while also conferring increased susceptibility to contemporary GBM therapy."xsd:string |
http://purl.uniprot.org/citations/31280432 | http://purl.org/dc/terms/identifier | "doi:10.1007/s11060-019-03230-y"xsd:string |
http://purl.uniprot.org/citations/31280432 | http://purl.uniprot.org/core/author | "Fernandes J."xsd:string |
http://purl.uniprot.org/citations/31280432 | http://purl.uniprot.org/core/author | "Chen P.A."xsd:string |
http://purl.uniprot.org/citations/31280432 | http://purl.uniprot.org/core/author | "van Landeghem F.K.H."xsd:string |
http://purl.uniprot.org/citations/31280432 | http://purl.uniprot.org/core/author | "Shrivastava G."xsd:string |
http://purl.uniprot.org/citations/31280432 | http://purl.uniprot.org/core/author | "Power C."xsd:string |
http://purl.uniprot.org/citations/31280432 | http://purl.uniprot.org/core/author | "Branton W.G."xsd:string |
http://purl.uniprot.org/citations/31280432 | http://purl.uniprot.org/core/author | "McKenzie B.A."xsd:string |
http://purl.uniprot.org/citations/31280432 | http://purl.uniprot.org/core/author | "Balcom E.F."xsd:string |
http://purl.uniprot.org/citations/31280432 | http://purl.uniprot.org/core/author | "Petruk K."xsd:string |
http://purl.uniprot.org/citations/31280432 | http://purl.uniprot.org/core/author | "Wheatley B.M."xsd:string |
http://purl.uniprot.org/citations/31280432 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
http://purl.uniprot.org/citations/31280432 | http://purl.uniprot.org/core/name | "J Neurooncol"xsd:string |
http://purl.uniprot.org/citations/31280432 | http://purl.uniprot.org/core/pages | "265-273"xsd:string |
http://purl.uniprot.org/citations/31280432 | http://purl.uniprot.org/core/title | "Absent in melanoma 2 regulates tumor cell proliferation in glioblastoma multiforme."xsd:string |
http://purl.uniprot.org/citations/31280432 | http://purl.uniprot.org/core/volume | "144"xsd:string |
http://purl.uniprot.org/citations/31280432 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/31280432 |
http://purl.uniprot.org/citations/31280432 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/31280432 |
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http://purl.uniprot.org/uniprot/#_O14862-mappedCitation-31280432 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31280432 |
http://purl.uniprot.org/uniprot/O14862 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/31280432 |
http://purl.uniprot.org/uniprot/L8E7Q5 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/31280432 |