| http://purl.uniprot.org/citations/31302248 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31302248 | http://www.w3.org/2000/01/rdf-schema#comment | "Yeast phosphatidylinositol transfer protein (PITP) Pdr17 is an essential component of the complex required for decarboxylation of phosphatidylserine (PS) to phosphatidylethanolamine (PE) at a non-mitochondrial location. According to current understanding, this process involves the transfer of PS from the endoplasmic reticulum to the Golgi/endosomes. We generated a Pdr17E237A, K269A mutant protein to better understand the mechanism by which Pdr17p participates in the processes connected to the decarboxylation of PS to PE. We show that the Pdr17E237A, K269A mutant protein is not capable of binding phosphatidylinositol (PI) using permeabilized human cells, but still retains the ability to transfer PI between two membrane compartments in vitro. We provide data together with molecular models showing that the mutations E237A and K269A changed only the lipid binding cavity of Pdr17p and not its surface properties. In contrast to Pdr16p, a close homologue, the ability of Pdr17p to bind PI is not required for its major cellular function in the inter-membrane transfer of PS. We hypothesize that these two closely related yeast PITPs, Pdr16p and Pdr17p, have evolved from a common ancestor. Pdr16p fulfills those role(s) in which the ability to bind and transfer PI is required, while Pdr17p appears to have adapted to a different role which does not require the high affinity binding of PI, although the protein retains the capacity to transfer PI. Our results indicate that PITPs function in complex ways in vivo and underscore the need to consider multiple PITP parameters when studying these proteins in vitro."xsd:string |
| http://purl.uniprot.org/citations/31302248 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbalip.2019.07.005"xsd:string |
| http://purl.uniprot.org/citations/31302248 | http://purl.uniprot.org/core/author | "Cockcroft S."xsd:string |
| http://purl.uniprot.org/citations/31302248 | http://purl.uniprot.org/core/author | "Kutejova E."xsd:string |
| http://purl.uniprot.org/citations/31302248 | http://purl.uniprot.org/core/author | "Griac P."xsd:string |
| http://purl.uniprot.org/citations/31302248 | http://purl.uniprot.org/core/author | "Pokorna L."xsd:string |
| http://purl.uniprot.org/citations/31302248 | http://purl.uniprot.org/core/author | "Tahotna D."xsd:string |
| http://purl.uniprot.org/citations/31302248 | http://purl.uniprot.org/core/author | "Holic R."xsd:string |
| http://purl.uniprot.org/citations/31302248 | http://purl.uniprot.org/core/author | "Bauer J.A."xsd:string |
| http://purl.uniprot.org/citations/31302248 | http://purl.uniprot.org/core/author | "Kotrasova V."xsd:string |
| http://purl.uniprot.org/citations/31302248 | http://purl.uniprot.org/core/author | "Pevala V."xsd:string |
| http://purl.uniprot.org/citations/31302248 | http://purl.uniprot.org/core/author | "Blunsom N.J."xsd:string |
| http://purl.uniprot.org/citations/31302248 | http://purl.uniprot.org/core/author | "Pevalova Z."xsd:string |
| http://purl.uniprot.org/citations/31302248 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/31302248 | http://purl.uniprot.org/core/name | "Biochim Biophys Acta Mol Cell Biol Lipids"xsd:string |
| http://purl.uniprot.org/citations/31302248 | http://purl.uniprot.org/core/pages | "1412-1421"xsd:string |
| http://purl.uniprot.org/citations/31302248 | http://purl.uniprot.org/core/title | "Yeast phosphatidylinositol transfer protein Pdr17 does not require high affinity phosphatidylinositol binding for its cellular function."xsd:string |
| http://purl.uniprot.org/citations/31302248 | http://purl.uniprot.org/core/volume | "1864"xsd:string |
| http://purl.uniprot.org/citations/31302248 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/31302248 |
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| http://purl.uniprot.org/uniprot/A0A6A5Q872 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/31302248 |
| http://purl.uniprot.org/uniprot/P53844 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/31302248 |