| http://purl.uniprot.org/citations/31381990 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31381990 | http://www.w3.org/2000/01/rdf-schema#comment | "Recombinant expression of human membrane proteins in large quantities remains a major challenge. Expression host is an important variable to screen for high-level production of membrane proteins. Using the green fluorescent protein (GFP) as a reporter, we screened the expression of a human multi-pass membrane protein called sterol Δ8-Δ7 isomerase in three different hosts: Escherichia coli, Saccharomyces cerevisiae, and Pichia pastoris. The expression of the His-tagged isomerase was exceptionally high in P. pastoris, reaching ~200 mg L-1 in standard flasks, and ~1,000 mg L-1 in condensed culture that mimics fermentation. The heterogeneously expressed isomerase could be extracted fully with dodecyl maltoside, and the solubilized protein in the form of GFP fusion showed a sharp and symmetric peak on fluorescence-detection size exclusion chromatography. Our work provides a useful source for the purification of the recombinant isomerase."xsd:string |
| http://purl.uniprot.org/citations/31381990 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.pep.2019.105463"xsd:string |
| http://purl.uniprot.org/citations/31381990 | http://purl.uniprot.org/core/author | "Li T."xsd:string |
| http://purl.uniprot.org/citations/31381990 | http://purl.uniprot.org/core/author | "Li D."xsd:string |
| http://purl.uniprot.org/citations/31381990 | http://purl.uniprot.org/core/author | "Tang Y."xsd:string |
| http://purl.uniprot.org/citations/31381990 | http://purl.uniprot.org/core/author | "Yao H."xsd:string |
| http://purl.uniprot.org/citations/31381990 | http://purl.uniprot.org/core/author | "Cai H."xsd:string |
| http://purl.uniprot.org/citations/31381990 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/31381990 | http://purl.uniprot.org/core/name | "Protein Expr Purif"xsd:string |
| http://purl.uniprot.org/citations/31381990 | http://purl.uniprot.org/core/pages | "105463"xsd:string |
| http://purl.uniprot.org/citations/31381990 | http://purl.uniprot.org/core/title | "High-level heterologous expression of the human transmembrane sterol Delta8,Delta7-isomerase in Pichia pastoris."xsd:string |
| http://purl.uniprot.org/citations/31381990 | http://purl.uniprot.org/core/volume | "164"xsd:string |
| http://purl.uniprot.org/citations/31381990 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/31381990 |
| http://purl.uniprot.org/citations/31381990 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/31381990 |
| http://purl.uniprot.org/uniprot/#_A0A024QYX0-mappedCitation-31381990 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31381990 |
| http://purl.uniprot.org/uniprot/#_Q15125-mappedCitation-31381990 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31381990 |
| http://purl.uniprot.org/uniprot/Q15125 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/31381990 |
| http://purl.uniprot.org/uniprot/A0A024QYX0 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/31381990 |