| http://purl.uniprot.org/citations/31392983 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31392983 | http://www.w3.org/2000/01/rdf-schema#comment | "Bacteriophage T4 middle promoters are activated through a process called σ appropriation, which requires the concerted effort of two T4-encoded transcription factors: AsiA and MotA. Despite extensive biochemical and genetic analyses, puzzle remains, in part, because of a lack of precise structural information for σ appropriation complex. Here, we report a single-particle cryo-electron microscopy (cryo-EM) structure of an intact σ appropriation complex, comprising AsiA, MotA, Escherichia coli RNA polymerase (RNAP), σ70 and a T4 middle promoter. As expected, AsiA binds to and remodels σ region 4 to prevent its contact with host promoters. Unexpectedly, AsiA undergoes a large conformational change, takes over the job of σ region 4 and provides an anchor point for the upstream double-stranded DNA. Because σ region 4 is conserved among bacteria, other transcription factors may use the same strategy to alter the landscape of transcription immediately. Together, the structure provides a foundation for understanding σ appropriation and transcription activation."xsd:string |
| http://purl.uniprot.org/citations/31392983 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gkz682"xsd:string |
| http://purl.uniprot.org/citations/31392983 | http://purl.uniprot.org/core/author | "Feng Y."xsd:string |
| http://purl.uniprot.org/citations/31392983 | http://purl.uniprot.org/core/author | "Shi J."xsd:string |
| http://purl.uniprot.org/citations/31392983 | http://purl.uniprot.org/core/author | "You L."xsd:string |
| http://purl.uniprot.org/citations/31392983 | http://purl.uniprot.org/core/author | "Zhang Y."xsd:string |
| http://purl.uniprot.org/citations/31392983 | http://purl.uniprot.org/core/author | "Zhao M."xsd:string |
| http://purl.uniprot.org/citations/31392983 | http://purl.uniprot.org/core/author | "Wen A."xsd:string |
| http://purl.uniprot.org/citations/31392983 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/31392983 | http://purl.uniprot.org/core/name | "Nucleic Acids Res"xsd:string |
| http://purl.uniprot.org/citations/31392983 | http://purl.uniprot.org/core/pages | "9423-9432"xsd:string |
| http://purl.uniprot.org/citations/31392983 | http://purl.uniprot.org/core/title | "Structural basis of sigma appropriation."xsd:string |
| http://purl.uniprot.org/citations/31392983 | http://purl.uniprot.org/core/volume | "47"xsd:string |
| http://purl.uniprot.org/citations/31392983 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/31392983 |
| http://purl.uniprot.org/citations/31392983 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/31392983 |
| http://purl.uniprot.org/uniprot/#_P00579-mappedCitation-31392983 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31392983 |
| http://purl.uniprot.org/uniprot/#_P22915-mappedCitation-31392983 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31392983 |
| http://purl.uniprot.org/uniprot/#_P32267-mappedCitation-31392983 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31392983 |
| http://purl.uniprot.org/uniprot/#_P0A7Z4-mappedCitation-31392983 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31392983 |
| http://purl.uniprot.org/uniprot/#_P0A800-mappedCitation-31392983 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31392983 |
| http://purl.uniprot.org/uniprot/#_P0A8T7-mappedCitation-31392983 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31392983 |
| http://purl.uniprot.org/uniprot/#_P0A8V2-mappedCitation-31392983 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31392983 |
| http://purl.uniprot.org/uniprot/P0A8T7 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/31392983 |
| http://purl.uniprot.org/uniprot/P22915 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/31392983 |