| http://purl.uniprot.org/citations/3139887 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/3139887 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/3139887 | http://www.w3.org/2000/01/rdf-schema#comment | "We have isolated and sequenced a yeast gene encoding a protein (Mr 24,875) very rich in serine (SRP) and alanine residues that accounted for 25% and 20% of the total amino acids, respectively. The SRP1 gene is highly expressed in culture conditions leading to glucose repression (Marguet & Lauquin, 1986), the amount of SRP1 mRNA representing about 1 to 2% of total poly(A)+ RNA. A repetitive structure of eight direct tandem repeats 36-base long, also reflected in the amino acid sequence, was found in the second half of the open reading frame. The consensus amino acid sequence of the repeat was Ser-Ser-Ser-Ala-Ala-Pro-Ser-Ser-Ser-Glu-Ala-Lys. Replacing the genomic copy of the cloned gene with a disrupted SRP1 gene indicated that the SRP1 gene was not essential for viability in yeast, but several SRP1-homologous sequences were found within the yeast genome, raising the possibility that the disrupted SRP1 gene is rescued by one of the other SRP-homologous sequences. Complete separation of yeast chromosomes by contour-clamped homogeneous field electrophoresis indicated that, apart from chromosome V, which carries the SRP1 gene, 12 chromosomes have SRP-related sequences with various degrees of homology. These sequences were located on chromosomes XV, VII and XI under stringent conditions of hybridization (tm -20 degrees C), and observed on chromosomes I, II, III, IV, VI, VIII, X, XI and XII, only under low-stringency conditions (tm -40 degrees C). Northern blot analysis of both the wild type and SRP1-disrupted strains indicated that along with SRP1 at least one more member of the SRP family was transcribed to a 0.7 kb (1 kb = 10(3) bases) polyadenylated RNA species clearly distinct from the SRP1-specific mRNA (1 kb long). Analyses of the SRP1 repeat domain suggested a model for the divergent evolution of the repeats in the SRP1 sequence."xsd:string |
| http://purl.uniprot.org/citations/3139887 | http://purl.org/dc/terms/identifier | "doi:10.1016/0022-2836(88)90278-1"xsd:string |
| http://purl.uniprot.org/citations/3139887 | http://purl.org/dc/terms/identifier | "doi:10.1016/0022-2836(88)90278-1"xsd:string |
| http://purl.uniprot.org/citations/3139887 | http://purl.uniprot.org/core/author | "Lauquin G.J.-M."xsd:string |
| http://purl.uniprot.org/citations/3139887 | http://purl.uniprot.org/core/author | "Lauquin G.J.-M."xsd:string |
| http://purl.uniprot.org/citations/3139887 | http://purl.uniprot.org/core/author | "Marguet D."xsd:string |
| http://purl.uniprot.org/citations/3139887 | http://purl.uniprot.org/core/author | "Marguet D."xsd:string |
| http://purl.uniprot.org/citations/3139887 | http://purl.uniprot.org/core/author | "Guo X.J."xsd:string |
| http://purl.uniprot.org/citations/3139887 | http://purl.uniprot.org/core/author | "Guo X.J."xsd:string |
| http://purl.uniprot.org/citations/3139887 | http://purl.uniprot.org/core/date | "1988"xsd:gYear |
| http://purl.uniprot.org/citations/3139887 | http://purl.uniprot.org/core/date | "1988"xsd:gYear |
| http://purl.uniprot.org/citations/3139887 | http://purl.uniprot.org/core/name | "J. Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/3139887 | http://purl.uniprot.org/core/name | "J. Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/3139887 | http://purl.uniprot.org/core/pages | "455-470"xsd:string |
| http://purl.uniprot.org/citations/3139887 | http://purl.uniprot.org/core/pages | "455-470"xsd:string |
| http://purl.uniprot.org/citations/3139887 | http://purl.uniprot.org/core/title | "Yeast gene SRP1 (serine-rich protein). Intragenic repeat structure and identification of a family of SRP1-related DNA sequences."xsd:string |
| http://purl.uniprot.org/citations/3139887 | http://purl.uniprot.org/core/title | "Yeast gene SRP1 (serine-rich protein). Intragenic repeat structure and identification of a family of SRP1-related DNA sequences."xsd:string |
| http://purl.uniprot.org/citations/3139887 | http://purl.uniprot.org/core/volume | "202"xsd:string |
| http://purl.uniprot.org/citations/3139887 | http://purl.uniprot.org/core/volume | "202"xsd:string |
| http://purl.uniprot.org/citations/3139887 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/3139887 |
| http://purl.uniprot.org/citations/3139887 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/3139887 |
| http://purl.uniprot.org/citations/3139887 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/3139887 |
| http://purl.uniprot.org/citations/3139887 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/3139887 |