| http://purl.uniprot.org/citations/31403468 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31403468 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31403468 | http://www.w3.org/2000/01/rdf-schema#comment | "We previously generated 32 rotavirus-specific (RV-specific) recombinant monoclonal antibodies (mAbs) derived from B cells isolated from human intestinal resections. Twenty-four of these mAbs were specific for the VP8* fragment of RV VP4, and most (20 of 24) were non-neutralizing when tested in the conventional MA104 cell-based assay. We reexamined the ability of these mAbs to neutralize RVs in human intestinal epithelial cells including ileal enteroids and HT-29 cells. Most (18 of 20) of the "non-neutralizing" VP8* mAbs efficiently neutralized human RV in HT-29 cells or enteroids. Serum RV neutralization titers in adults and infants were significantly higher in HT-29 than MA104 cells and adsorption of these sera with recombinant VP8* lowered the neutralization titers in HT-29 but not MA104 cells. VP8* mAbs also protected suckling mice from diarrhea in an in vivo challenge model. X-ray crystallographic analysis of one VP8* mAb (mAb9) in complex with human RV VP8* revealed that the mAb interaction site was distinct from the human histo-blood group antigen binding site. Since MA104 cells are the most commonly used cell line to detect anti-RV neutralization activity, these findings suggest that prior vaccine and other studies of human RV neutralization responses may have underestimated the contribution of VP8* antibodies to the overall neutralization titer."xsd:string |
| http://purl.uniprot.org/citations/31403468 | http://purl.org/dc/terms/identifier | "doi:10.1172/JCI128382"xsd:string |
| http://purl.uniprot.org/citations/31403468 | http://purl.org/dc/terms/identifier | "doi:10.1172/jci128382"xsd:string |
| http://purl.uniprot.org/citations/31403468 | http://purl.uniprot.org/core/author | "Prasad B.V.V."xsd:string |
| http://purl.uniprot.org/citations/31403468 | http://purl.uniprot.org/core/author | "Prasad B.V.V."xsd:string |
| http://purl.uniprot.org/citations/31403468 | http://purl.uniprot.org/core/author | "Song Y."xsd:string |
| http://purl.uniprot.org/citations/31403468 | http://purl.uniprot.org/core/author | "Song Y."xsd:string |
| http://purl.uniprot.org/citations/31403468 | http://purl.uniprot.org/core/author | "Sankaran B."xsd:string |
| http://purl.uniprot.org/citations/31403468 | http://purl.uniprot.org/core/author | "Sankaran B."xsd:string |
| http://purl.uniprot.org/citations/31403468 | http://purl.uniprot.org/core/author | "Zhao B."xsd:string |
| http://purl.uniprot.org/citations/31403468 | http://purl.uniprot.org/core/author | "Zhao B."xsd:string |
| http://purl.uniprot.org/citations/31403468 | http://purl.uniprot.org/core/author | "Hu L."xsd:string |
| http://purl.uniprot.org/citations/31403468 | http://purl.uniprot.org/core/author | "Hu L."xsd:string |
| http://purl.uniprot.org/citations/31403468 | http://purl.uniprot.org/core/author | "Ding S."xsd:string |
| http://purl.uniprot.org/citations/31403468 | http://purl.uniprot.org/core/author | "Ding S."xsd:string |
| http://purl.uniprot.org/citations/31403468 | http://purl.uniprot.org/core/author | "Feng N."xsd:string |
| http://purl.uniprot.org/citations/31403468 | http://purl.uniprot.org/core/author | "Feng N."xsd:string |
| http://purl.uniprot.org/citations/31403468 | http://purl.uniprot.org/core/author | "Greenberg H.B."xsd:string |
| http://purl.uniprot.org/citations/31403468 | http://purl.uniprot.org/core/author | "Greenberg H.B."xsd:string |
| http://purl.uniprot.org/citations/31403468 | http://purl.uniprot.org/core/author | "Ramani S."xsd:string |
| http://purl.uniprot.org/citations/31403468 | http://purl.uniprot.org/core/author | "Ramani S."xsd:string |
| http://purl.uniprot.org/citations/31403468 | http://purl.uniprot.org/core/author | "Sanyal M."xsd:string |
| http://purl.uniprot.org/citations/31403468 | http://purl.uniprot.org/core/author | "Sanyal M."xsd:string |