| http://purl.uniprot.org/citations/31406020 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31406020 | http://www.w3.org/2000/01/rdf-schema#comment | "Protein kinase D (PKD) is an essential Ser/Thr kinase in animals and controls a variety of diverse cellular functions, including vesicle trafficking and mitogenesis. PKD is activated by recruitment to membranes containing the lipid second messenger diacylglycerol (DAG) and subsequent phosphorylation of its activation loop. Here, we report the crystal structure of the PKD N terminus at 2.2 Å resolution containing a previously unannotated ubiquitin-like domain (ULD), which serves as a dimerization domain. A single point mutation in the dimerization interface of the ULD not only abrogated dimerization in cells but also prevented PKD activation loop phosphorylation upon DAG production. We further show that the kinase domain of PKD dimerizes in a concentration-dependent manner and autophosphorylates on a single residue in its activation loop. We also provide evidence that PKD is expressed at concentrations 2 orders of magnitude below the ULD dissociation constant in mammalian cells. We therefore propose a new model for PKD activation in which the production of DAG leads to the local accumulation of PKD at the membrane, which drives ULD-mediated dimerization and subsequent trans-autophosphorylation of the kinase domain."xsd:string |
| http://purl.uniprot.org/citations/31406020 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.ra119.008713"xsd:string |
| http://purl.uniprot.org/citations/31406020 | http://purl.uniprot.org/core/author | "Hartl M."xsd:string |
| http://purl.uniprot.org/citations/31406020 | http://purl.uniprot.org/core/author | "Leonard T.A."xsd:string |
| http://purl.uniprot.org/citations/31406020 | http://purl.uniprot.org/core/author | "Gossenreiter T."xsd:string |
| http://purl.uniprot.org/citations/31406020 | http://purl.uniprot.org/core/author | "Elsner D.J."xsd:string |
| http://purl.uniprot.org/citations/31406020 | http://purl.uniprot.org/core/author | "Siess K.M."xsd:string |
| http://purl.uniprot.org/citations/31406020 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/31406020 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/31406020 | http://purl.uniprot.org/core/pages | "14422-14441"xsd:string |
| http://purl.uniprot.org/citations/31406020 | http://purl.uniprot.org/core/title | "A ubiquitin-like domain controls protein kinase D dimerization and activation by trans-autophosphorylation."xsd:string |
| http://purl.uniprot.org/citations/31406020 | http://purl.uniprot.org/core/volume | "294"xsd:string |
| http://purl.uniprot.org/citations/31406020 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/31406020 |
| http://purl.uniprot.org/citations/31406020 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/31406020 |
| http://purl.uniprot.org/uniprot/#_Q15139-mappedCitation-31406020 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31406020 |
| http://purl.uniprot.org/uniprot/#_Q9XUJ7-mappedCitation-31406020 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31406020 |
| http://purl.uniprot.org/uniprot/Q15139 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/31406020 |
| http://purl.uniprot.org/uniprot/Q9XUJ7 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/31406020 |