| http://purl.uniprot.org/citations/31476383 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31476383 | http://www.w3.org/2000/01/rdf-schema#comment | "Mammalian ovarian follicular atresia is a complex and fine-regulated biological process with active involvement of connective tissue growth factor (CTGF). The emergence of studies of endogenous non-coding RNAs has raised a new aspect for exploration of the regulatory mechanisms involved in follicular atresia. Here, we aimed to illustrate a circRNA involved in the CTGF regulatory pathway during the apoptosis and follicular atresia of pig granulosa cells (GCs). We first detected a decreased expression pattern of CTGF during follicular atresia using IHC, FISH and qRT-PCR and confirmed the anti-apoptosis effect of CTGF in GCs in vitro by CTGF siRNA knockdown. Then, we used a dual luciferase activity assay to demonstrate CTGF as a direct functional target of miR-10a-5p, which was upregulated in atresic follicles and promoted the apoptosis of GCs in vitro. The negative effect of miR-10a-5p on GC viability was confirmed by cell cycle assays, cell proliferation/apoptosis assays and the WB detection of marker proteins. More importantly, we identified a novel circRNA, termed circINHA, that was downregulated during atresia in ovarian follicles, and we confirmed a direct interaction between miR-10a-5p and circINHA. Finally, we demonstrated that circINHA promoted GCs proliferation and inhibited GCs apoptosis via CTGF as a competing endogenous RNA (ceRNA) that directly bound to miR-10a-5p. Taken together, this study provides evidence for the circINHA/miR-10a-5p/CTGF regulatory pathway in follicular GC apoptosis and provides novel insights into the role of circRNAs in the modulation of ovarian physiological functions."xsd:string |
| http://purl.uniprot.org/citations/31476383 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbagrm.2019.194420"xsd:string |
| http://purl.uniprot.org/citations/31476383 | http://purl.uniprot.org/core/author | "Huang L."xsd:string |
| http://purl.uniprot.org/citations/31476383 | http://purl.uniprot.org/core/author | "Liu H."xsd:string |
| http://purl.uniprot.org/citations/31476383 | http://purl.uniprot.org/core/author | "Li Q."xsd:string |
| http://purl.uniprot.org/citations/31476383 | http://purl.uniprot.org/core/author | "Ma M."xsd:string |
| http://purl.uniprot.org/citations/31476383 | http://purl.uniprot.org/core/author | "Zhang J."xsd:string |
| http://purl.uniprot.org/citations/31476383 | http://purl.uniprot.org/core/author | "Guo T."xsd:string |
| http://purl.uniprot.org/citations/31476383 | http://purl.uniprot.org/core/author | "Du X."xsd:string |
| http://purl.uniprot.org/citations/31476383 | http://purl.uniprot.org/core/author | "Pan Z."xsd:string |
| http://purl.uniprot.org/citations/31476383 | http://purl.uniprot.org/core/author | "Li Q.'"xsd:string |
| http://purl.uniprot.org/citations/31476383 | http://purl.uniprot.org/core/author | "Yao W."xsd:string |
| http://purl.uniprot.org/citations/31476383 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/31476383 | http://purl.uniprot.org/core/name | "Biochim Biophys Acta Gene Regul Mech"xsd:string |
| http://purl.uniprot.org/citations/31476383 | http://purl.uniprot.org/core/pages | "194420"xsd:string |
| http://purl.uniprot.org/citations/31476383 | http://purl.uniprot.org/core/title | "CircINHA resists granulosa cell apoptosis by upregulating CTGF as a ceRNA of miR-10a-5p in pig ovarian follicles."xsd:string |
| http://purl.uniprot.org/citations/31476383 | http://purl.uniprot.org/core/volume | "1862"xsd:string |
| http://purl.uniprot.org/citations/31476383 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/31476383 |
| http://purl.uniprot.org/citations/31476383 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/31476383 |
| http://purl.uniprot.org/uniprot/#_O19113-mappedCitation-31476383 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31476383 |
| http://purl.uniprot.org/uniprot/#_Q15I73-mappedCitation-31476383 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31476383 |
| http://purl.uniprot.org/uniprot/#_O97765-mappedCitation-31476383 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31476383 |
| http://purl.uniprot.org/uniprot/O19113 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/31476383 |
| http://purl.uniprot.org/uniprot/Q15I73 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/31476383 |