| http://purl.uniprot.org/citations/31492634 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31492634 | http://www.w3.org/2000/01/rdf-schema#comment | "DNA double-strand breaks (DSBs) threaten genome stability throughout life and are linked to tumorigenesis in humans. To initiate DSB repair by end joining or homologous recombination, the Mre11-nuclease Rad50-ATPase complex detects and processes diverse and obstructed DNA ends, but a structural mechanism is still lacking. Here we report cryo-EM structures of the E. coli Mre11-Rad50 homolog SbcCD in resting and DNA-bound cutting states. In the resting state, Mre11's nuclease is blocked by ATP-Rad50, and the Rad50 coiled coils appear flexible. Upon DNA binding, the two coiled coils zip up into a rod and, together with the Rad50 nucleotide-binding domains, form a clamp around dsDNA. Mre11 moves to the side of Rad50, binds the DNA end, and assembles a DNA cutting channel for the nuclease reactions. The structures reveal how Mre11-Rad50 can detect and process diverse DNA ends and uncover a clamping and gating function for the coiled coils."xsd:string |
| http://purl.uniprot.org/citations/31492634 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.molcel.2019.07.035"xsd:string |
| http://purl.uniprot.org/citations/31492634 | http://purl.uniprot.org/core/author | "Hopfner K.P."xsd:string |
| http://purl.uniprot.org/citations/31492634 | http://purl.uniprot.org/core/author | "Kessler B."xsd:string |
| http://purl.uniprot.org/citations/31492634 | http://purl.uniprot.org/core/author | "Bartho J."xsd:string |
| http://purl.uniprot.org/citations/31492634 | http://purl.uniprot.org/core/author | "Lammens K."xsd:string |
| http://purl.uniprot.org/citations/31492634 | http://purl.uniprot.org/core/author | "Alt A."xsd:string |
| http://purl.uniprot.org/citations/31492634 | http://purl.uniprot.org/core/author | "Kashammer L."xsd:string |
| http://purl.uniprot.org/citations/31492634 | http://purl.uniprot.org/core/author | "Gut F."xsd:string |
| http://purl.uniprot.org/citations/31492634 | http://purl.uniprot.org/core/author | "Saathoff J.H."xsd:string |
| http://purl.uniprot.org/citations/31492634 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/31492634 | http://purl.uniprot.org/core/name | "Mol Cell"xsd:string |
| http://purl.uniprot.org/citations/31492634 | http://purl.uniprot.org/core/pages | "382-394.e6"xsd:string |
| http://purl.uniprot.org/citations/31492634 | http://purl.uniprot.org/core/title | "Mechanism of DNA End Sensing and Processing by the Mre11-Rad50 Complex."xsd:string |
| http://purl.uniprot.org/citations/31492634 | http://purl.uniprot.org/core/volume | "76"xsd:string |
| http://purl.uniprot.org/citations/31492634 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/31492634 |
| http://purl.uniprot.org/citations/31492634 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/31492634 |
| http://purl.uniprot.org/uniprot/#_P0AG76-mappedCitation-31492634 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31492634 |
| http://purl.uniprot.org/uniprot/#_P13458-mappedCitation-31492634 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31492634 |
| http://purl.uniprot.org/uniprot/P0AG76 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/31492634 |
| http://purl.uniprot.org/uniprot/P13458 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/31492634 |