| http://purl.uniprot.org/citations/3149564 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/3149564 | http://www.w3.org/2000/01/rdf-schema#comment | "Three separate mechanisms of cell-cell adhesion have been shown to appear at different stages of development in Dictyostelium discoideum. During the first few hours of development, the cells synthesize and accumulate a glycoprotein of 24,000 daltons (gp24) that is positioned in the membrane. The time of appearance of gp24 correlates exactly with the time of appearance of cell-cell adhesion in two strains in which temporal control varies by several hours. Antibodies specific to gp24 are able to block cell-cell adhesion during the first few hours of development but not during later development. By 8 hr of development, another glycoprotein, gp80, that is not recognized by antibodies to gp24 accumulates on the surface of cells. This membrane protein mediates an independent adhesion mechanism during the aggregation stage that is resistant to 10 mM EDTA. Antibodies specific to gp80 can block EDTA-resistant adhesion during this stage. During subsequent development, gp80 is removed from the cell surface and replaced by another adhesion mechanism that is insensitive to antibodies to either gp24 or gp80. A lambda gt11 expression vector carrying a Dictyostelium cDNA insert was isolated that directs the synthesis of a fusion protein recognized by antibodies specific to gp24. This cDNA was used to probe a genomic library. A clone carrying a 1.4-kb insert of genomic DNA was recognized by the cDNA and shown to hybridize to a 0.7-kb mRNA that accumulates early in development. This unusually small RNA could code for the small protein, gp24. Southern analysis of restriction fragments generated by various enzymes on Dictyostelium DNA with both the cDNA and genomic clones indicated the presence of two tandem copies of the gene. This may account for the failure to recover mutations resulting in the lack of gp24. Mutations have been recovered that result in the lack of accumulation of gp80, and cells carrying these mutations have been shown to be missing the second adhesion mechanism. These mutant strains are able to complete development because the other adhesion mechanisms are not impaired. Sequential addition of adhesion mechanisms provides a means for the formation of multicellular organisms from previously solitary cells."xsd:string |
| http://purl.uniprot.org/citations/3149564 | http://purl.org/dc/terms/identifier | "doi:10.1002/dvg.1020090431"xsd:string |
| http://purl.uniprot.org/citations/3149564 | http://purl.uniprot.org/core/author | "Loomis W.F."xsd:string |
| http://purl.uniprot.org/citations/3149564 | http://purl.uniprot.org/core/date | "1988"xsd:gYear |
| http://purl.uniprot.org/citations/3149564 | http://purl.uniprot.org/core/name | "Dev Genet"xsd:string |
| http://purl.uniprot.org/citations/3149564 | http://purl.uniprot.org/core/pages | "549-559"xsd:string |
| http://purl.uniprot.org/citations/3149564 | http://purl.uniprot.org/core/title | "Cell-cell adhesion in Dictyostelium discoideum."xsd:string |
| http://purl.uniprot.org/citations/3149564 | http://purl.uniprot.org/core/volume | "9"xsd:string |
| http://purl.uniprot.org/citations/3149564 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/3149564 |
| http://purl.uniprot.org/citations/3149564 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/3149564 |
| http://purl.uniprot.org/uniprot/#_P08796-mappedCitation-3149564 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/3149564 |
| http://purl.uniprot.org/uniprot/#_P54657-mappedCitation-3149564 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/3149564 |
| http://purl.uniprot.org/uniprot/P54657 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/3149564 |
| http://purl.uniprot.org/uniprot/P08796 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/3149564 |