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http://purl.uniprot.org/citations/31520422http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
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Background

microRNA (miR)-218-5p is involved in cigarette smoke-induced airway inflammation. In our earlier asthma epithelial miRNA profiling data, miR-218-5p was the top 2 down-regulated miRNA. We hypothesize that miR-218-5p plays a role in asthma airway inflammation.

Objective

To unveil the role of miR-218-5p and its target gene in asthma airway inflammation.

Methods

We measured miR-218-5p expression in bronchial brushings of asthma patients (n = 50) and healthy controls (n = 15), and analysed the correlations between miR-218-5p expression and airway eosinophilia. We examined whether CTNND2 was a target of miR-218-5p, and the expression of 12 catenin family members in bronchial brushings, in cultured human bronchial epithelial (HBE) cells and BEAS-2B cells. We explored the role of miR-218-5p-CTNND2 pathway using a murine model of allergic airway inflammation.

Results

Epithelial miR-218-5p expression was significantly decreased and negatively correlated with eosinophils in induced sputum and bronchial biopsies, and other type 2 biomarkers in asthma patients. We verified that CTNND2 (encoding δ-catenin) was a target of miR-218-5p. Remarkably, CTNND2 was the most significantly up-regulated catenin compared with the other 11 catenin family members in bronchial brushings of asthma patients, IL-13-stimulated HBE and BEAS-2B cells. Moreover, epithelial CTNND2 expression positively correlated with airway eosinophilia in asthma. Airway mmu-miR-218-5p expression was also decreased, and Ctnnd2 expression was increased in a murine model of allergic airway inflammation. Intriguingly, mmu-miR-218-5p overexpression suppressed airway hyperresponsiveness, eosinophilic airway inflammation and Ctnnd2 up-regulation in the mouse model. Finally, perturbation of miR-218-5p or CTNND2 expression significantly altered chemokine CCL26 expression in the cell cultures and the mouse model.

Conclusions and clinical relevance

Epithelial miR-218-5p plays a protective role in eosinophilic airway inflammation via targeting CTNND2, a novel catenin in asthma, and suppressing chemokine CCL26 expression."xsd:string
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http://purl.uniprot.org/citations/31520422http://purl.uniprot.org/core/author"Chen D."xsd:string
http://purl.uniprot.org/citations/31520422http://purl.uniprot.org/core/author"Chang C."xsd:string
http://purl.uniprot.org/citations/31520422http://purl.uniprot.org/core/author"Feng Y."xsd:string
http://purl.uniprot.org/citations/31520422http://purl.uniprot.org/core/author"Liang Y."xsd:string
http://purl.uniprot.org/citations/31520422http://purl.uniprot.org/core/author"Wu W."xsd:string
http://purl.uniprot.org/citations/31520422http://purl.uniprot.org/core/author"Zhen G."xsd:string
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http://purl.uniprot.org/citations/31520422http://purl.uniprot.org/core/volume"50"xsd:string
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