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http://purl.uniprot.org/citations/31570430http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/31570430http://www.w3.org/2000/01/rdf-schema#comment"

Background/aim

The mechanism responsible for B-cell translocation gene 1 (BTG1) down-regulation in breast carcinoma remains unknown. We examined the BTG1 expression status in breast carcinoma cells and investigated the mechanism underlying the observed alterations.

Materials and methods

Four breast carcinoma cell lines (SK-BR-3, MDA-MB-231, T-47D, and MCF-7), and one normal mammary epithelial cell line (MCF-10A) were analyzed. BTG1 expression was examined using quantitative reverse transcription polymerase chain reaction (PCR) and western blot. Methylation status of the BTG1 promoter was analyzed using methylation-specific PCR (MSP). To investigate the effect of methylation on BTG1, the cells were treated with a demethylating agent.

Results

The carcinoma cells expressed significantly lower levels of BTG1 mRNA and protein than normal cells. The BTG1 promoter was highly methylated in the carcinoma cells. 5-aza-2-deoxycytidine significantly restored BTG1 expression.

Conclusion

Down-regulation of BTG1 expression through epigenetic repression is involved in mammary carcinogenesis. BTG1 is a potential diagnostic marker and therapeutic target for breast carcinoma."xsd:string
http://purl.uniprot.org/citations/31570430http://purl.org/dc/terms/identifier"doi:10.21873/anticanres.13729"xsd:string
http://purl.uniprot.org/citations/31570430http://purl.uniprot.org/core/author"Kim H.S."xsd:string
http://purl.uniprot.org/citations/31570430http://purl.uniprot.org/core/author"Kim S.H."xsd:string
http://purl.uniprot.org/citations/31570430http://purl.uniprot.org/core/author"Song S.Y."xsd:string
http://purl.uniprot.org/citations/31570430http://purl.uniprot.org/core/author"Woo H.Y."xsd:string
http://purl.uniprot.org/citations/31570430http://purl.uniprot.org/core/author"DO S.I."xsd:string
http://purl.uniprot.org/citations/31570430http://purl.uniprot.org/core/date"2019"xsd:gYear
http://purl.uniprot.org/citations/31570430http://purl.uniprot.org/core/name"Anticancer Res"xsd:string
http://purl.uniprot.org/citations/31570430http://purl.uniprot.org/core/pages"5361-5367"xsd:string
http://purl.uniprot.org/citations/31570430http://purl.uniprot.org/core/title"Promoter Methylation Down-regulates B-cell Translocation Gene 1 Expression in Breast Carcinoma."xsd:string
http://purl.uniprot.org/citations/31570430http://purl.uniprot.org/core/volume"39"xsd:string
http://purl.uniprot.org/citations/31570430http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/31570430
http://purl.uniprot.org/citations/31570430http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/31570430
http://purl.uniprot.org/uniprot/#_P62324-mappedCitation-31570430http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/31570430
http://purl.uniprot.org/uniprot/#_Q6IBC8-mappedCitation-31570430http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/31570430
http://purl.uniprot.org/uniprot/P62324http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/31570430
http://purl.uniprot.org/uniprot/Q6IBC8http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/31570430