| http://purl.uniprot.org/citations/31603944 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31603944 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31603944 | http://www.w3.org/2000/01/rdf-schema#comment | "The carboxysome is a bacterial micro-compartment (BMC) subtype that encapsulates enzymatic activities necessary for carbon fixation. Carboxysome shells are composed of a relatively complex cocktail of proteins, their precise number and identity being species dependent. Shell components can be classified in two structural families, the most abundant class associating as hexamers (BMC-H) that are supposed to be major players for regulating shell permeability. Up to recently, these proteins were proposed to associate as homo-oligomers. Genomic data, however, demonstrated the existence of paralogs coding for multiple shell subunits. Here, we studied cross-association compatibilities among BMC-H CcmK proteins of Synechocystis sp. PCC6803. Co-expression in Escherichia coli proved a consistent formation of hetero-hexamers combining CcmK1 and CcmK2 or, remarkably, CcmK3 and CcmK4 subunits. Unlike CcmK1/K2 hetero-hexamers, the stoichiometry of incorporation of CcmK3 in associations with CcmK4 was low. Cross-interactions implicating other combinations were weak, highlighting a structural segregation of the two groups that could relate to gene organization. Sequence analysis and structural models permitted the localization of interactions that would favor formation of CcmK3/K4 hetero-hexamers. The crystallization of these CcmK3/K4 associations conducted to the elucidation of a structure corresponding to the CcmK4 homo-hexamer. Yet, subunit exchange could not be demonstrated in vitro. Biophysical measurements showed that hetero-hexamers are thermally less stable than homo-hexamers, and impeded in forming larger assemblies. These novel findings are discussed within the context of reported data to propose a functional scenario in which minor CcmK3/K4 incorporation in shells would introduce sufficient local disorder as to allow shell remodeling necessary to adapt rapidly to environmental changes."xsd:string |
| http://purl.uniprot.org/citations/31603944 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0223877"xsd:string |
| http://purl.uniprot.org/citations/31603944 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0223877"xsd:string |
| http://purl.uniprot.org/citations/31603944 | http://purl.uniprot.org/core/author | "Mourey L."xsd:string |
| http://purl.uniprot.org/citations/31603944 | http://purl.uniprot.org/core/author | "Mourey L."xsd:string |
| http://purl.uniprot.org/citations/31603944 | http://purl.uniprot.org/core/author | "Aubry N."xsd:string |
| http://purl.uniprot.org/citations/31603944 | http://purl.uniprot.org/core/author | "Aubry N."xsd:string |
| http://purl.uniprot.org/citations/31603944 | http://purl.uniprot.org/core/author | "Garcia-Alles L.F."xsd:string |
| http://purl.uniprot.org/citations/31603944 | http://purl.uniprot.org/core/author | "Garcia-Alles L.F."xsd:string |
| http://purl.uniprot.org/citations/31603944 | http://purl.uniprot.org/core/author | "Lesniewska E."xsd:string |
| http://purl.uniprot.org/citations/31603944 | http://purl.uniprot.org/core/author | "Lesniewska E."xsd:string |
| http://purl.uniprot.org/citations/31603944 | http://purl.uniprot.org/core/author | "Maveyraud L."xsd:string |
| http://purl.uniprot.org/citations/31603944 | http://purl.uniprot.org/core/author | "Maveyraud L."xsd:string |
| http://purl.uniprot.org/citations/31603944 | http://purl.uniprot.org/core/author | "Root K."xsd:string |
| http://purl.uniprot.org/citations/31603944 | http://purl.uniprot.org/core/author | "Root K."xsd:string |
| http://purl.uniprot.org/citations/31603944 | http://purl.uniprot.org/core/author | "Truan G."xsd:string |
| http://purl.uniprot.org/citations/31603944 | http://purl.uniprot.org/core/author | "Truan G."xsd:string |
| http://purl.uniprot.org/citations/31603944 | http://purl.uniprot.org/core/author | "Zenobi R."xsd:string |
| http://purl.uniprot.org/citations/31603944 | http://purl.uniprot.org/core/author | "Zenobi R."xsd:string |
| http://purl.uniprot.org/citations/31603944 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/31603944 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/31603944 | http://purl.uniprot.org/core/name | "PLoS ONE"xsd:string |
| http://purl.uniprot.org/citations/31603944 | http://purl.uniprot.org/core/name | "PLoS ONE"xsd:string |