| http://purl.uniprot.org/citations/31662984 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31662984 | http://www.w3.org/2000/01/rdf-schema#comment | "The use of 5-fluorouracil (5-FU) has been proven benefits, but it also has adverse events in colorectal cancer (CRC) chemotherapy. In this study, we explored the mechanism of 5-FU resistance by bioinformatics analysis of the NCBI public dataset series GSE81005. Fifteen hub genes were screened out of 582 different expressed genes. Modules of the hub genes in protein-protein interaction networks gathered to TOP2α showed a decrease in HCT-8 cells but an increase in 5-FU-resistant HCT-8/5-FU cells with 5-FU exposure. Downregulation of TOP2α with siRNA or miR-494 transfection resulted in an increase of cytotoxicity and decrease of cell colonies to 5-FU for HCT-8/5-FU cells. Moreover, we found that an ethanol extract of Spica Prunellae (EESP), which is a traditional Chinese medicine with clinically beneficial effects in various cancers, was able to enhance the sensitivity of 5-FU in HCT-8/5-FU cells and partly reverse the 5-FU resistance effect. It significantly helped suppress cell growth and induced cell apoptosis in HCT-8/5-FU cells with the expression of TOP2α being significantly suppressed, which increased by 5-FU. Consistently, miR-494, which reportedly regulates TOP2α, exhibited reverse trends in EESP/5-FU combination treatment. These results suggested that Spica Prunellae may be beneficial in the treatment of 5-FU-resistant CRC patients."xsd:string |
| http://purl.uniprot.org/citations/31662984 | http://purl.org/dc/terms/identifier | "doi:10.1155/2019/5953619"xsd:string |
| http://purl.uniprot.org/citations/31662984 | http://purl.uniprot.org/core/author | "Fang Y."xsd:string |
| http://purl.uniprot.org/citations/31662984 | http://purl.uniprot.org/core/author | "Lin J."xsd:string |
| http://purl.uniprot.org/citations/31662984 | http://purl.uniprot.org/core/author | "Peng J."xsd:string |
| http://purl.uniprot.org/citations/31662984 | http://purl.uniprot.org/core/author | "Zhang L."xsd:string |
| http://purl.uniprot.org/citations/31662984 | http://purl.uniprot.org/core/author | "Yang C."xsd:string |
| http://purl.uniprot.org/citations/31662984 | http://purl.uniprot.org/core/author | "Wei L."xsd:string |
| http://purl.uniprot.org/citations/31662984 | http://purl.uniprot.org/core/author | "Zhao J."xsd:string |
| http://purl.uniprot.org/citations/31662984 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/31662984 | http://purl.uniprot.org/core/name | "Biomed Res Int"xsd:string |
| http://purl.uniprot.org/citations/31662984 | http://purl.uniprot.org/core/pages | "5953619"xsd:string |
| http://purl.uniprot.org/citations/31662984 | http://purl.uniprot.org/core/title | "Spica Prunellae Extract Enhances Fluorouracil Sensitivity of 5-Fluorouracil-Resistant Human Colon Carcinoma HCT-8/5-FU Cells via TOP2pii>alphapi/i> and miR-494."xsd:string |
| http://purl.uniprot.org/citations/31662984 | http://purl.uniprot.org/core/volume | "2019"xsd:string |
| http://purl.uniprot.org/citations/31662984 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/31662984 |
| http://purl.uniprot.org/citations/31662984 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/31662984 |
| http://purl.uniprot.org/uniprot/#_A0A4D6UXC9-mappedCitation-31662984 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31662984 |
| http://purl.uniprot.org/uniprot/#_P11388-mappedCitation-31662984 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31662984 |
| http://purl.uniprot.org/uniprot/P11388 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/31662984 |
| http://purl.uniprot.org/uniprot/A0A4D6UXC9 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/31662984 |