| http://purl.uniprot.org/citations/31663248 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31663248 | http://www.w3.org/2000/01/rdf-schema#comment | "The spatial and temporal dynamics of cell contractility plays a key role in tissue morphogenesis, wound healing, and cancer invasion. Here, we report a simple optochemical method to induce cell contractions in vivo during Drosophila morphogenesis at single-cell resolution. We employed the photolabile Ca2+ chelator o-nitrophenyl EGTA to induce bursts of intracellular free Ca2+ by laser photolysis in the epithelial tissue. Ca2+ bursts appear within seconds and are restricted to individual target cells. Cell contraction reliably followed within a minute, causing an approximately 50% drop in the cross-sectional area. Increased Ca2+ levels are reversible, and the target cells further participated in tissue morphogenesis. Depending on Rho kinase (ROCK) activity but not RhoGEF2, cell contractions are paralleled with non-muscle myosin II accumulation in the apico-medial cortex, indicating that Ca2+ bursts trigger non-muscle myosin II activation. Our approach can be, in principle, adapted to many experimental systems and species, as no specific genetic elements are required."xsd:string |
| http://purl.uniprot.org/citations/31663248 | http://purl.org/dc/terms/identifier | "doi:10.15252/embr.201947755"xsd:string |
| http://purl.uniprot.org/citations/31663248 | http://purl.uniprot.org/core/author | "Haring M."xsd:string |
| http://purl.uniprot.org/citations/31663248 | http://purl.uniprot.org/core/author | "Lin B."xsd:string |
| http://purl.uniprot.org/citations/31663248 | http://purl.uniprot.org/core/author | "Lv Z."xsd:string |
| http://purl.uniprot.org/citations/31663248 | http://purl.uniprot.org/core/author | "Kong D."xsd:string |
| http://purl.uniprot.org/citations/31663248 | http://purl.uniprot.org/core/author | "Grosshans J."xsd:string |
| http://purl.uniprot.org/citations/31663248 | http://purl.uniprot.org/core/author | "Wolf F."xsd:string |
| http://purl.uniprot.org/citations/31663248 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/31663248 | http://purl.uniprot.org/core/name | "EMBO Rep"xsd:string |
| http://purl.uniprot.org/citations/31663248 | http://purl.uniprot.org/core/pages | "e47755"xsd:string |
| http://purl.uniprot.org/citations/31663248 | http://purl.uniprot.org/core/title | "In vivo optochemical control of cell contractility at single-cell resolution."xsd:string |
| http://purl.uniprot.org/citations/31663248 | http://purl.uniprot.org/core/volume | "20"xsd:string |
| http://purl.uniprot.org/citations/31663248 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/31663248 |
| http://purl.uniprot.org/citations/31663248 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/31663248 |
| http://purl.uniprot.org/uniprot/#_A0A0B4LFF8-mappedCitation-31663248 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31663248 |
| http://purl.uniprot.org/uniprot/#_A0A0B4K7T7-mappedCitation-31663248 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31663248 |
| http://purl.uniprot.org/uniprot/#_A0A0B4KGY5-mappedCitation-31663248 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31663248 |
| http://purl.uniprot.org/uniprot/#_F6J1D0-mappedCitation-31663248 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31663248 |
| http://purl.uniprot.org/uniprot/#_A1ZAN6-mappedCitation-31663248 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31663248 |
| http://purl.uniprot.org/uniprot/#_A1ZAN7-mappedCitation-31663248 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31663248 |
| http://purl.uniprot.org/uniprot/#_O44113-mappedCitation-31663248 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31663248 |
| http://purl.uniprot.org/uniprot/#_O44381-mappedCitation-31663248 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31663248 |
| http://purl.uniprot.org/uniprot/#_Q24584-mappedCitation-31663248 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31663248 |