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http://purl.uniprot.org/citations/31669240http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/31669240http://www.w3.org/2000/01/rdf-schema#comment"

Objective

We aimed to explore the expression level and biological function of miR-145-5p in preeclampsia (PE).

Methods

The differentially expressed miRNA/mRNA between normal placentas and PE placentas were screened using the GSE84260 and GSE73374 datasets from the Gene Expression Omnibus Database. The expression of miR-145-5p in PE placentas was detected by qRT-PCR. The CCK-8 assay, wound healing and transwell were carried out to determine the cell growth, migration and invasion when miR-145-5p was overexpressed or inhibited. The real-time quantitative PCR (qRT-PCR), Western Blot and dual-luciferase reporter assays were conducted to preliminarily explore possible mechanisms.

Results

A total of 33 miRNAs were found significantly differentially expressed in PE patients, 19 were significantly upregulated and 14 were significantly downregulated. The relative miR-145-5p expression was lower in PE placentas than normal placentas. The viability and invasion were suppressed when miR-145-5p was inhibited in trophoblasts cells, while miR-145-5p overexpression promoted the effectiveness. In addition, mRNA and protein expression of FLT1 in HTR-8/SVneo cell was also downregulated with miR-145-5p overexpression, suggesting that FLT1 is the target gene of miR-145-5p. Consistent with miR-145-5p overexpression, the mRNA and protein expression of FLT1 also were upregulated with miR-145-5p interference. Furthermore, the expression of miR-145-5p was regulated by the Hypoxic conditions.

Conclusions

In conclusion, the results showed miR-145-5p may participate in PE development by affecting the proliferation and invasion of trophoblast cells. This is a new perspective to understand the etiology and pathogenesis of PE, which may provide a new breakthrough for the early prediction and diagnosis of PE."xsd:string
http://purl.uniprot.org/citations/31669240http://purl.org/dc/terms/identifier"doi:10.1016/j.lfs.2019.117008"xsd:string
http://purl.uniprot.org/citations/31669240http://purl.uniprot.org/core/author"Fan Y."xsd:string
http://purl.uniprot.org/citations/31669240http://purl.uniprot.org/core/author"Li C."xsd:string
http://purl.uniprot.org/citations/31669240http://purl.uniprot.org/core/author"Lu X."xsd:string
http://purl.uniprot.org/citations/31669240http://purl.uniprot.org/core/author"Lv Y."xsd:string
http://purl.uniprot.org/citations/31669240http://purl.uniprot.org/core/author"Wang L."xsd:string
http://purl.uniprot.org/citations/31669240http://purl.uniprot.org/core/author"Wu L."xsd:string
http://purl.uniprot.org/citations/31669240http://purl.uniprot.org/core/author"Ji X."xsd:string
http://purl.uniprot.org/citations/31669240http://purl.uniprot.org/core/author"Ding H."xsd:string
http://purl.uniprot.org/citations/31669240http://purl.uniprot.org/core/author"Meng L."xsd:string
http://purl.uniprot.org/citations/31669240http://purl.uniprot.org/core/author"Lv M."xsd:string
http://purl.uniprot.org/citations/31669240http://purl.uniprot.org/core/author"Long W."xsd:string
http://purl.uniprot.org/citations/31669240http://purl.uniprot.org/core/date"2019"xsd:gYear
http://purl.uniprot.org/citations/31669240http://purl.uniprot.org/core/name"Life Sci"xsd:string
http://purl.uniprot.org/citations/31669240http://purl.uniprot.org/core/pages"117008"xsd:string
http://purl.uniprot.org/citations/31669240http://purl.uniprot.org/core/title"miR-145-5p promotes trophoblast cell growth and invasion by targeting FLT1."xsd:string
http://purl.uniprot.org/citations/31669240http://purl.uniprot.org/core/volume"239"xsd:string
http://purl.uniprot.org/citations/31669240http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/31669240
http://purl.uniprot.org/citations/31669240http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/31669240
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http://purl.uniprot.org/uniprot/#_H9N1E8-mappedCitation-31669240http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/31669240
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