http://purl.uniprot.org/citations/3170489 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/3170489 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/3170489 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
http://purl.uniprot.org/citations/3170489 | http://www.w3.org/2000/01/rdf-schema#comment | "A 2,598-base-pair (bp) SalI-HincII DNA fragment has been cloned which codes for vanillate demethylase, the enzyme responsible for the demethylation of vanillate (3-methoxy-4-hydroxybenzoate) to protocatechuate (3,4-dihydroxybenzoate). Complementation and insertional inactivation experiments have shown that this fragment carries two genes (vanA and vanB) which are predominantly cotranscribed from a promoter upstream of vanA. Nucleotide sequencing of the SalI-HincII fragment confirmed the genetic data: two open reading frames of 987 and 942 bp were present in the transcribed orientation. These had a very high G + C content in the third base of each codon, which is characteristic of Pseudomonas chromosomal genes. Expression of the genes in Escherichia coli with the T7 RNA polymerase-promoter system gave rise to two polypeptides of 36 and 33 kilodaltons which could be identified by deletion analysis as the products of vanA and vanB, respectively. A search of the protein sequence data bank indicated that the vanB gene product was related to the ferredoxin family."xsd:string |
http://purl.uniprot.org/citations/3170489 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.170.10.4924-4930.1988"xsd:string |
http://purl.uniprot.org/citations/3170489 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.170.10.4924-4930.1988"xsd:string |
http://purl.uniprot.org/citations/3170489 | http://purl.uniprot.org/core/author | "Brunel F."xsd:string |
http://purl.uniprot.org/citations/3170489 | http://purl.uniprot.org/core/author | "Brunel F."xsd:string |
http://purl.uniprot.org/citations/3170489 | http://purl.uniprot.org/core/author | "Davison J."xsd:string |
http://purl.uniprot.org/citations/3170489 | http://purl.uniprot.org/core/author | "Davison J."xsd:string |
http://purl.uniprot.org/citations/3170489 | http://purl.uniprot.org/core/date | "1988"xsd:gYear |
http://purl.uniprot.org/citations/3170489 | http://purl.uniprot.org/core/date | "1988"xsd:gYear |
http://purl.uniprot.org/citations/3170489 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
http://purl.uniprot.org/citations/3170489 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
http://purl.uniprot.org/citations/3170489 | http://purl.uniprot.org/core/pages | "4924-4930"xsd:string |
http://purl.uniprot.org/citations/3170489 | http://purl.uniprot.org/core/pages | "4924-4930"xsd:string |
http://purl.uniprot.org/citations/3170489 | http://purl.uniprot.org/core/title | "Cloning and sequencing of Pseudomonas genes encoding vanillate demethylase."xsd:string |
http://purl.uniprot.org/citations/3170489 | http://purl.uniprot.org/core/title | "Cloning and sequencing of Pseudomonas genes encoding vanillate demethylase."xsd:string |
http://purl.uniprot.org/citations/3170489 | http://purl.uniprot.org/core/volume | "170"xsd:string |
http://purl.uniprot.org/citations/3170489 | http://purl.uniprot.org/core/volume | "170"xsd:string |
http://purl.uniprot.org/citations/3170489 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/3170489 |
http://purl.uniprot.org/citations/3170489 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/3170489 |
http://purl.uniprot.org/citations/3170489 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/3170489 |
http://purl.uniprot.org/citations/3170489 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/3170489 |
http://purl.uniprot.org/citations/3170489 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/3170489 |