| http://purl.uniprot.org/citations/31722958 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31722958 | http://www.w3.org/2000/01/rdf-schema#comment | "Construction of a genome-wide transgenic UAS-cDNA/ORF library in Drosophila based on the binary GAL4/UAS system has been severely hampered by technical difficulties, although genome-wide cDNA or ORF resources of Drosophila, human, and mouse have been publicly available for more than a decade. Here, we developed a new method named CRISPR-based modular assembly (CRISPRmass) for the high-throughput construction of a genome-wide UAS-cDNA/ORF library from publicly available cDNA/ORF resources. Through cleavage of shared vector sequences of cDNA/ORF plasmids by CRISPR/Cas9 and subsequent insertion of UAS modules by Gibson assembly, the procedure of construction of such a library by CRISPRmass is standardized as massively parallel two-step test tube reactions before bacterial transformation. Using CRISPRmass, we generated 5551 UAS-cDNA/ORF constructs covering 83% of the Drosophila genes conserved in humans in the Drosophila Genomics Resource Center (DGRC) Gold Collection, and among them, 5518 were generated within 3 mo by three people. Our results show that CRISPRmass allows modulization, simplicity, efficiency, and adaptability in the generation of a genome-wide UAS-cDNA/ORF plasmid library by using publicly available cDNA/ORF resources. CRISPRmass can be applied to editing various genome-wide libraries in general and is an alternative to Gateway technology in high-throughput plasmid library editing. Furthermore, the more than 5500 UAS-cDNA/ORF plasmids of Drosophila genes serve as a powerful resource for gain-of-function (GOF) screening in cultured cells and for generation of a transgenic UAS-cDNA/ORF library in Drosophila."xsd:string |
| http://purl.uniprot.org/citations/31722958 | http://purl.org/dc/terms/identifier | "doi:10.1101/gr.250811.119"xsd:string |
| http://purl.uniprot.org/citations/31722958 | http://purl.uniprot.org/core/author | "Wang J."xsd:string |
| http://purl.uniprot.org/citations/31722958 | http://purl.uniprot.org/core/author | "Zhao Y."xsd:string |
| http://purl.uniprot.org/citations/31722958 | http://purl.uniprot.org/core/author | "Xue W."xsd:string |
| http://purl.uniprot.org/citations/31722958 | http://purl.uniprot.org/core/author | "Wei P."xsd:string |
| http://purl.uniprot.org/citations/31722958 | http://purl.uniprot.org/core/author | "Ning G."xsd:string |
| http://purl.uniprot.org/citations/31722958 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
| http://purl.uniprot.org/citations/31722958 | http://purl.uniprot.org/core/name | "Genome Res"xsd:string |
| http://purl.uniprot.org/citations/31722958 | http://purl.uniprot.org/core/pages | "95-106"xsd:string |
| http://purl.uniprot.org/citations/31722958 | http://purl.uniprot.org/core/title | "CRISPR-based modular assembly of a UAS-cDNA/ORF plasmid library for more than 5500 Drosophila genes conserved in humans."xsd:string |
| http://purl.uniprot.org/citations/31722958 | http://purl.uniprot.org/core/volume | "30"xsd:string |
| http://purl.uniprot.org/citations/31722958 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/31722958 |
| http://purl.uniprot.org/citations/31722958 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/31722958 |
| http://purl.uniprot.org/uniprot/#_A0A0C4DH96-mappedCitation-31722958 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31722958 |
| http://purl.uniprot.org/uniprot/#_A0A0C4DHA5-mappedCitation-31722958 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31722958 |
| http://purl.uniprot.org/uniprot/#_A0A0C4DHB5-mappedCitation-31722958 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31722958 |
| http://purl.uniprot.org/uniprot/#_A0A0C4DHB8-mappedCitation-31722958 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31722958 |
| http://purl.uniprot.org/uniprot/#_A0A0C4DHC2-mappedCitation-31722958 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31722958 |
| http://purl.uniprot.org/uniprot/#_A0A0C4DHC8-mappedCitation-31722958 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31722958 |
| http://purl.uniprot.org/uniprot/#_A0A0C4DHD1-mappedCitation-31722958 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31722958 |
| http://purl.uniprot.org/uniprot/#_A0A0B4KFF1-mappedCitation-31722958 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31722958 |
| http://purl.uniprot.org/uniprot/#_A0A0B4KFG0-mappedCitation-31722958 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31722958 |
| http://purl.uniprot.org/uniprot/#_A0A0B4KFG1-mappedCitation-31722958 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31722958 |