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http://purl.uniprot.org/citations/31762230http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/31762230http://www.w3.org/2000/01/rdf-schema#comment"

Objective

To investigate the mitochondrial translocation of hypoxia inducible factor-3α (HIF-3α) under normoxia and hypoxia and its physiological and pathological meanings.

Methods

① After hypoxic (1%O2) or DMOG, CoCl2 treatments mimicking the hypoxic treatment, Western blot and immunofluorescence were used to examine the HIF-3α expression in mitochondria of HeLa and ACHN cells, respectively. ②The protease sensitivity experiment was used to explore the sub-organelle localization of HIF-3α in mitochondria. ③Western blot was used to examine mitochondrial HIF-3α in the normal mouse tissues and human liver carcinoma tissues.

Results

① In HeLa and ACHN cells, HIF-3α translocated to mitochondria under normoxia and hypoxia, and its mitochondrial expression was higher under hypoxia; ②The protease sensitivity of HIF-3α was similar to proteins locating in the mitochondrial outer membrane; ③Mitochondrial HIF-3α expressed in multiple normal mouse tissues; The expression of mitochondrial HIF-3α was higher in human liver carcinoma tissues than the normal and adjacent tissues.

Conclusions

HIF-3α translocated to mitochondrial outer membrane under both normoxia and hypoxia, and hypoxia could up-regulated HIF-3α mitochondrial translocation. Meanwhile, the phenomenon may be involved in the process of liver carcinoma."xsd:string
http://purl.uniprot.org/citations/31762230http://purl.uniprot.org/core/author"Feng L."xsd:string
http://purl.uniprot.org/citations/31762230http://purl.uniprot.org/core/author"Li L."xsd:string
http://purl.uniprot.org/citations/31762230http://purl.uniprot.org/core/author"Long D."xsd:string
http://purl.uniprot.org/citations/31762230http://purl.uniprot.org/core/author"Li Y.P."xsd:string
http://purl.uniprot.org/citations/31762230http://purl.uniprot.org/core/author"Li H.S."xsd:string
http://purl.uniprot.org/citations/31762230http://purl.uniprot.org/core/author"Li S.F."xsd:string
http://purl.uniprot.org/citations/31762230http://purl.uniprot.org/core/author"Zhou Y.N."xsd:string
http://purl.uniprot.org/citations/31762230http://purl.uniprot.org/core/date"2019"xsd:gYear
http://purl.uniprot.org/citations/31762230http://purl.uniprot.org/core/name"Sichuan Da Xue Xue Bao Yi Xue Ban"xsd:string
http://purl.uniprot.org/citations/31762230http://purl.uniprot.org/core/pages"635-642"xsd:string
http://purl.uniprot.org/citations/31762230http://purl.uniprot.org/core/title"[Expression and Significance of HIF-3alpha in Mitochondria]."xsd:string
http://purl.uniprot.org/citations/31762230http://purl.uniprot.org/core/volume"50"xsd:string
http://purl.uniprot.org/citations/31762230http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/31762230
http://purl.uniprot.org/citations/31762230http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/31762230
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