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Background

Cigarette smoke plays an important role in the pathogenesis of chronic obstructive pulmonary disease (COPD). Recently, elevated serotonin (5-HT) levels were found in the plasma of COPD patients. The role of 5-HT and its receptors in airway inflammation and remodeling induced by cigarette smoke is unclear.

Methods

BALB/c mice received the 5-HTR2A inhibitor ketanserin, the 5-HTR2B inhibitor RS-127445 or the natural 5-HTR2A/2B inhibitor quercetin intraperitoneally, then were exposed to cigarette smoke for 6 or 12 weeks. Control mice received placebo and were exposed to room air or cigarette smoke. Mice were sacrificed and bronchial alveolar lavage fluid (BALF) and lung tissue samples were collected.

Results

Immunohistochemistry and western blot confirmed an increase in both 5-HTR2A and 5-HTR2B expression in mouse lungs after exposure to cigarette smoke for 6 and 12 weeks. Cigarette smoke induced accumulation of macrophages and neutrophils and increased levels of inflammatory cytokines, including IL-1β and TNF-ɑ, in BALF and lung tissue; these effects were inhibited by ketanserin, RS-127445 and quercetin. Pretreatment with 5-HT receptor antagonists suppressed the goblet cell hyperplasia induced by 6- or 12-week exposure to cigarette smoke, based on Alcian blue-periodic acid Schiff staining. After 12 weeks of cigarette smoke exposure, Masson's staining showed fibrosis surrounding the mouse airways, and inhibitor pretreatment significantly attenuated the thickening and collagen deposition around the small airways.

Conclusions

Our results suggest that cigarette smoke-induced airway inflammation and small airway remodeling are partially mediated by 5-HTR2A and 5-HTR2B, which could be a new therapeutic target for airway remodeling in COPD."xsd:string
http://purl.uniprot.org/citations/31787571http://purl.org/dc/terms/identifier"doi:10.1016/j.intimp.2019.106036"xsd:string
http://purl.uniprot.org/citations/31787571http://purl.uniprot.org/core/author"Chen L."xsd:string
http://purl.uniprot.org/citations/31787571http://purl.uniprot.org/core/author"Dong J."xsd:string
http://purl.uniprot.org/citations/31787571http://purl.uniprot.org/core/author"Li Y."xsd:string
http://purl.uniprot.org/citations/31787571http://purl.uniprot.org/core/author"Shen Y."xsd:string
http://purl.uniprot.org/citations/31787571http://purl.uniprot.org/core/author"Wu Y."xsd:string
http://purl.uniprot.org/citations/31787571http://purl.uniprot.org/core/author"Wang H."xsd:string
http://purl.uniprot.org/citations/31787571http://purl.uniprot.org/core/author"Yang T."xsd:string
http://purl.uniprot.org/citations/31787571http://purl.uniprot.org/core/author"Zeng Z."xsd:string
http://purl.uniprot.org/citations/31787571http://purl.uniprot.org/core/author"Wan C."xsd:string
http://purl.uniprot.org/citations/31787571http://purl.uniprot.org/core/author"Wen F."xsd:string
http://purl.uniprot.org/citations/31787571http://purl.uniprot.org/core/date"2020"xsd:gYear
http://purl.uniprot.org/citations/31787571http://purl.uniprot.org/core/name"Int Immunopharmacol"xsd:string
http://purl.uniprot.org/citations/31787571http://purl.uniprot.org/core/pages"106036"xsd:string
http://purl.uniprot.org/citations/31787571http://purl.uniprot.org/core/title"Serotonin receptors 5-HTR2A and 5-HTR2B are involved in cigarette smoke-induced airway inflammation, mucus hypersecretion and airway remodeling in mice."xsd:string
http://purl.uniprot.org/citations/31787571http://purl.uniprot.org/core/volume"81"xsd:string
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