| http://purl.uniprot.org/citations/31792309 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31792309 | http://www.w3.org/2000/01/rdf-schema#comment | "Persistent organochlorine pollutants (POPs) gradually accumulate in the human organism due to their presence in the environment. Some studies have described a correlation between the level of POPs in the human body and the incidence of diabetes, but we know little about the direct effect of POPs on pancreatic beta-cells. We exposed pancreatic beta-cells INS1E to non-lethal concentrations of p,p'-DDT (1,1'-(2,2,2-Trichloroethane-1,1-diyl)bis(4-chlorobenzene)) and p,p'-DDE (1,1'-(2,2-dichloroethene-1,1-diyl)bis(4-chlorobenzene)) for 1 month, and assessed changes in protein expression and the intracellular insulin level. 2-D electrophoresis revealed 6 proteins with changed expression in cells exposed to p,p'-DDT or p,p'-DDE. One of the detected proteins - vitamin D-binding protein (VDBP) - was upregulated in both cells exposed to p,p'-DDT, and cells exposed to p,p'-DDE. Both exposures to pollutants reduced the intracellular level of insulin mRNA, proinsulin, and insulin monomer; p,p'-DDT also slightly reduced the level of hexameric insulin. Overexpression of VDBP caused by the stable transfection of beta-cells with the gene for VDBP decreased both the proinsulin and hexameric insulin level in beta-cells similarly to the reduction detected in cells exposed to p,p'-DDT. Our data suggest that in the cells exposed to p,p'-DDT and p,p'-DDE, the increased VDBP protein level decreased the proinsulin expression in an unknown mechanism."xsd:string |
| http://purl.uniprot.org/citations/31792309 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41598-019-54579-z"xsd:string |
| http://purl.uniprot.org/citations/31792309 | http://purl.uniprot.org/core/author | "Halada P."xsd:string |
| http://purl.uniprot.org/citations/31792309 | http://purl.uniprot.org/core/author | "Daniel P."xsd:string |
| http://purl.uniprot.org/citations/31792309 | http://purl.uniprot.org/core/author | "Sramek J."xsd:string |
| http://purl.uniprot.org/citations/31792309 | http://purl.uniprot.org/core/author | "Pavlikova N."xsd:string |
| http://purl.uniprot.org/citations/31792309 | http://purl.uniprot.org/core/author | "Kovar J."xsd:string |
| http://purl.uniprot.org/citations/31792309 | http://purl.uniprot.org/core/author | "Jelinek M."xsd:string |
| http://purl.uniprot.org/citations/31792309 | http://purl.uniprot.org/core/author | "Nemcova V."xsd:string |
| http://purl.uniprot.org/citations/31792309 | http://purl.uniprot.org/core/author | "Balusikova K."xsd:string |
| http://purl.uniprot.org/citations/31792309 | http://purl.uniprot.org/core/author | "Sramkova V."xsd:string |
| http://purl.uniprot.org/citations/31792309 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
| http://purl.uniprot.org/citations/31792309 | http://purl.uniprot.org/core/name | "Sci Rep"xsd:string |
| http://purl.uniprot.org/citations/31792309 | http://purl.uniprot.org/core/pages | "18026"xsd:string |
| http://purl.uniprot.org/citations/31792309 | http://purl.uniprot.org/core/title | "Upregulation of vitamin D-binding protein is associated with changes in insulin production in pancreatic beta-cells exposed to p,p'-DDT and p,p'-DDE."xsd:string |
| http://purl.uniprot.org/citations/31792309 | http://purl.uniprot.org/core/volume | "9"xsd:string |
| http://purl.uniprot.org/citations/31792309 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/31792309 |
| http://purl.uniprot.org/citations/31792309 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/31792309 |
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| http://purl.uniprot.org/uniprot/#_A6KKH3-mappedCitation-31792309 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31792309 |
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| http://purl.uniprot.org/uniprot/#_P04276-mappedCitation-31792309 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31792309 |
| http://purl.uniprot.org/uniprot/P04276 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/31792309 |