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http://purl.uniprot.org/citations/31801690http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/31801690http://www.w3.org/2000/01/rdf-schema#comment"

Purpose

To investigate the effect of miR-200c/PAI-2 on macrophage polarization into M2-type TAMs in TNBC.

Methods and materials

PAI-2 expression in MDA-MB-231con, MDA-MB-231miR-200ab and MDA-MB-231miR-200c breast cancer cells was evaluated by RT-PCR and immunofluorescence (IF), while the expression of the TAM marker F4/80 and the M2-type TAM marker CD206 in MDA-MB-231con, MDA-MB-231miR-200c and MDA-MB-231miR-200c/siPAI-2 mouse lung metastatic tumor tissues was examined with immunohistochemistry (IHC). The effects of RAW264.7 cells on MDA-MB-231con, MDA-MB-231miR-200c and MDA-MB-231miR-200c/siPAI-2 were examined by transwell co-culture. CD206 expression in RAW264.7 cells were confirmed by immunostaining. The level of PAI-2 and IL-10 in the co-culture supernatants were assessed using ELISA.

Results

1. RT-PCR and IF analysis showed that PAI-2 was upregulated in MDA-MB-231miR-200c cells. 2. IHC assays analysis showed that the numbers of F4/80 and CD206 positive cells were increased in MDA-MB-231miR-200c tumor tissues, while in MDA-MB-231miR-200c/siPAI-2 tumor tissues were decreased. 3. Transwell co-culture assays analysis showed that MDA-MB-231miR-200c cells significantly promoted the cell migration ability compared with the control group, while knockdown PAI-2 significantly inhibited the cell migration ability (P < 0.05). 4. Transwell co-culture and immunostaining assays analysis showed that overexpression miR-200c in MDA-MB-231 cell line increased the CD206 expression in RAW264.7 cells, while knockdown PAI-2 decreased. 5. ELISA assays analysis showed that miR-200c-mediated MDA-MB-231 cells significantly increased the secretion of PAI-2 and IL-10, while decreased the secretion of PAI-2 and IL-10 in MDA-MB-231 miR-200c/siPAI-2 cells.

Conclusions

miR-200c promotes the malignant progressions of TNBC by PAI-2 upregulation and M2 phenotype macrophages polarization."xsd:string
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http://purl.uniprot.org/citations/31801690http://purl.uniprot.org/core/author"Li C."xsd:string
http://purl.uniprot.org/citations/31801690http://purl.uniprot.org/core/author"Wang Y."xsd:string
http://purl.uniprot.org/citations/31801690http://purl.uniprot.org/core/author"Zhang R."xsd:string
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http://purl.uniprot.org/citations/31801690http://purl.uniprot.org/core/author"Zhu G."xsd:string
http://purl.uniprot.org/citations/31801690http://purl.uniprot.org/core/author"Jin T."xsd:string
http://purl.uniprot.org/citations/31801690http://purl.uniprot.org/core/author"Meng Z."xsd:string
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http://purl.uniprot.org/citations/31801690http://purl.uniprot.org/core/title"miR-200c/PAI-2 promotes the progression of triple negative breast cancer via M1/M2 polarization induction of macrophage."xsd:string
http://purl.uniprot.org/citations/31801690http://purl.uniprot.org/core/volume"81"xsd:string
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