http://purl.uniprot.org/citations/31828174 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/31828174 | http://www.w3.org/2000/01/rdf-schema#comment | "ObjectiveTo investigate the transplantation effect of bone marrow mesenchymal stem cells (MSCs) on the expression of interlukin-22 (IL-22) and RANKL in collagen-induced arthritis (CIA) rats.Methods32 CIA models were established. 16 CIA rats were transplanted with MSCs, and others were used as nontreatment CIA controls. The concentrations of IL-22 and RANKL in serum were detected by ELISA and those in synovial tissue of rats' joints by immunohistochemical staining. In addition, the expression of RANKL mRNA was measured by RT-PCR in the fibroblast-like synoviocytes (FLSs), cultured with IL-22 in vitro, which were delivered from the joints of CIA rats treated with or without MSCs.ResultsThe transplantation of MSCs into CIA rats relieved the destruction of joints, measured by AI score, X-ray, and histopathology. MSCs also reduced the expression of IL-22 and RANKL in serum by ELISA (P < 0.001) and similarly in FLSs by immunohistochemical staining. In vitro, IL-22 induced significantly the expression of RANKL mRNA in cultured FLSs in a dose-dependent manner, whereas this induction was significantly reduced in FLSs derived from CIA rats transplanted with MSCs (normal controls: F = 79.33, P < 0.001; CIA controls: F = 712.72, P < 0.001; and CIA-MSC rats: F = 139.04, P < 0.001).ConclusionOur results suggest that the transplantation of MSCs can reduce the expression of RANKL in vivo by downregulating the levels of IL-22, thereby ameliorating the degree of RA bone destruction. This study provides a theoretical basis for a potential therapy of RA with MSCs, and IL-22 and RANKL may become two new targets to treat RA."xsd:string |
http://purl.uniprot.org/citations/31828174 | http://purl.org/dc/terms/identifier | "doi:10.1155/2019/8459281"xsd:string |
http://purl.uniprot.org/citations/31828174 | http://purl.uniprot.org/core/author | "Gao C."xsd:string |
http://purl.uniprot.org/citations/31828174 | http://purl.uniprot.org/core/author | "Li F."xsd:string |
http://purl.uniprot.org/citations/31828174 | http://purl.uniprot.org/core/author | "Li X."xsd:string |
http://purl.uniprot.org/citations/31828174 | http://purl.uniprot.org/core/author | "Liu G."xsd:string |
http://purl.uniprot.org/citations/31828174 | http://purl.uniprot.org/core/author | "Li X.'"xsd:string |
http://purl.uniprot.org/citations/31828174 | http://purl.uniprot.org/core/date | "2019"xsd:gYear |
http://purl.uniprot.org/citations/31828174 | http://purl.uniprot.org/core/name | "J Immunol Res"xsd:string |
http://purl.uniprot.org/citations/31828174 | http://purl.uniprot.org/core/pages | "8459281"xsd:string |
http://purl.uniprot.org/citations/31828174 | http://purl.uniprot.org/core/title | "Bone Marrow Mesenchymal Stem Cells Decrease the Expression of RANKL in Collagen-Induced Arthritis Rats via Reducing the Levels of IL-22."xsd:string |
http://purl.uniprot.org/citations/31828174 | http://purl.uniprot.org/core/volume | "2019"xsd:string |
http://purl.uniprot.org/citations/31828174 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/31828174 |
http://purl.uniprot.org/citations/31828174 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/31828174 |
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http://purl.uniprot.org/uniprot/#_Q9ESE2-mappedCitation-31828174 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31828174 |
http://purl.uniprot.org/uniprot/Q9ESE2 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/31828174 |
http://purl.uniprot.org/uniprot/A6HTX4 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/31828174 |