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http://purl.uniprot.org/citations/31832002http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/31832002http://www.w3.org/2000/01/rdf-schema#comment"

Background

Gastric cancer (GC) has become a serious threat to people's health. Accumulative evidence reveals that dysregulation of numerous microRNAs (miRNAs) has been found during malignant formation. So far, the role of microRNA-760 (miR-760) in the development of GC is largely unknown.

Aim

To measure the expression level of miR-760 in GC and investigate its role in gastric tumorigenesis.

Methods

Real-time quantitative polymerase chain reaction and Western blot analysis were used to measure the expression of miR-760 and G-protein-coupled receptor kinase interacting protein-1 (GIT1). Cell growth was detected by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) and cell colony formation assays. Apoptosis was assessed by flow cytometric analysis. The relationship between miR-760 and GIT1 was verified by luciferase reporter assay.

Results

The results showed that the expression of miR-760 was decreased in GC and associated with poor clinical outcomes in GC patients. Furthermore, miR-760 restrained cell proliferation and cell colony formation and induced apoptosis in GC cells. In addition, miR-760 directly targeted GIT1 and negatively regulated its expression in GC. GIT1 was upregulated in GC and predicted a worse prognosis in GC patients. We also found that upregulation of GIT1 weakened the inhibitory effect of miR-760 in GC.

Conclusion

In conclusion, miR-760 targets GIT1 to inhibit cell growth and promote apoptosis in GC cells. Our data demonstrate that miR-760 may be a potential target for the treatment of GC."xsd:string
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http://purl.uniprot.org/citations/31832002http://purl.uniprot.org/core/author"Wang Y."xsd:string
http://purl.uniprot.org/citations/31832002http://purl.uniprot.org/core/author"Ge L."xsd:string
http://purl.uniprot.org/citations/31832002http://purl.uniprot.org/core/author"Liu S.S."xsd:string
http://purl.uniprot.org/citations/31832002http://purl.uniprot.org/core/author"Duan Q.H."xsd:string
http://purl.uniprot.org/citations/31832002http://purl.uniprot.org/core/author"Liu G.J."xsd:string
http://purl.uniprot.org/citations/31832002http://purl.uniprot.org/core/date"2019"xsd:gYear
http://purl.uniprot.org/citations/31832002http://purl.uniprot.org/core/name"World J Gastroenterol"xsd:string
http://purl.uniprot.org/citations/31832002http://purl.uniprot.org/core/pages"6619-6633"xsd:string
http://purl.uniprot.org/citations/31832002http://purl.uniprot.org/core/title"MicroRNA-760 acts as a tumor suppressor in gastric cancer development via inhibiting G-protein-coupled receptor kinase interacting protein-1 transcription."xsd:string
http://purl.uniprot.org/citations/31832002http://purl.uniprot.org/core/volume"25"xsd:string
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