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http://purl.uniprot.org/citations/31858324http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/31858324http://www.w3.org/2000/01/rdf-schema#comment"

Background

Hepatocellular carcinoma (HCC) is a serious threat to human lives and is usually diagnosed at the late stages. Recently, there has been a rapid advancement in the treatment options for HCC, but novel therapeutic targets are still needed, especially for precision medicine.

Aims

We aimed to investigate the involvement of non-coding RNA RP11-81H3.2 in HCC.

Methods

The expression of RP11-81H3.2 was examined in the blood samples of HCC patients, and in the human HCC cell lines, including HepG2, Smmc-7721, and Huh7. Cell proliferation was determined using the CCK-8 and EdU assay, and cell invasion and migration were determined using the transwell/wound healing assay. The effects of RP11-81H3.2 knockdown on in vivo tumor growth were evaluated utilizing the nude mice HepG2 tumor xenograft model.

Results

Here, we have identified a long non-coding RNA, RP11-81H3.2, which is enriched in HCC and can promote its proliferation, migration, and invasion both in vitro and in vivo. In addition, our results showed that RP11-81H3.2 binds to and regulate miR-490-3p expression in the HCC cells. Moreover, we found that RP11-81H3.2 regulates the expression of TNKS2 via miR-490-3p. Further, we found that RP11-81H3.2 and miR-490-3p form a regulatory loop; the release of RP11-81H3.2 leads to the suppression of miR-490-3p expression, thus, further enhancing the expression of RP11-81H3.2.

Conclusions

Our data have provided a novel target for the diagnosis and treatment of HCC, and sheds light on the lncRNA-miRNA regulatory nexus that can control the HCC related pathogenesis."xsd:string
http://purl.uniprot.org/citations/31858324http://purl.org/dc/terms/identifier"doi:10.1007/s10620-019-06007-5"xsd:string
http://purl.uniprot.org/citations/31858324http://purl.uniprot.org/core/author"Chen W."xsd:string
http://purl.uniprot.org/citations/31858324http://purl.uniprot.org/core/author"Li K."xsd:string
http://purl.uniprot.org/citations/31858324http://purl.uniprot.org/core/author"Zhu K."xsd:string
http://purl.uniprot.org/citations/31858324http://purl.uniprot.org/core/author"Li W.H."xsd:string
http://purl.uniprot.org/citations/31858324http://purl.uniprot.org/core/author"Yan R."xsd:string
http://purl.uniprot.org/citations/31858324http://purl.uniprot.org/core/author"Dang C.X."xsd:string
http://purl.uniprot.org/citations/31858324http://purl.uniprot.org/core/author"Cai Q.C."xsd:string
http://purl.uniprot.org/citations/31858324http://purl.uniprot.org/core/date"2020"xsd:gYear
http://purl.uniprot.org/citations/31858324http://purl.uniprot.org/core/name"Dig Dis Sci"xsd:string
http://purl.uniprot.org/citations/31858324http://purl.uniprot.org/core/pages"2949-2958"xsd:string
http://purl.uniprot.org/citations/31858324http://purl.uniprot.org/core/title"RP11-81H3.2 Acts as an Oncogene via microRNA-490-3p Inhibition and Consequential Tankyrase 2 Up-Regulation in Hepatocellular Carcinoma."xsd:string
http://purl.uniprot.org/citations/31858324http://purl.uniprot.org/core/volume"65"xsd:string
http://purl.uniprot.org/citations/31858324http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/31858324
http://purl.uniprot.org/citations/31858324http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/31858324
http://purl.uniprot.org/uniprot/#_Q9H2K2-mappedCitation-31858324http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/31858324
http://purl.uniprot.org/uniprot/Q9H2K2http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/31858324