| http://purl.uniprot.org/citations/31923473 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31923473 | http://www.w3.org/2000/01/rdf-schema#comment | "The triggering receptor expressed on myeloid cells (TREM) protein family forms a class of type I transmembrane proteins expressed in immune cells that play important roles in innate and adaptive immune responses. The TREM family member TREM-like transcript 1 (TLT-1, also TREML1) is expressed in megakaryocytes and packaged into platelet granules. TLT-1 binds fibrinogen and plays a role in bleeding initiated by inflammatory insults. Here, we describe a proteomics screen that maps the TLT-1 interactome in resting and activated human platelets. Several identified TLT-1 interactors are involved in cell adhesion and migration, as well as platelet activation. Select interactors, including β3-integrin, RACK1, GRB2, and Rabs 5A, 7, and 11A, were additionally characterized in co-immunoprecipitation/immunoblotting experiments. Finally, several phosphorylation sites were found on immunoprecipitated TLT-1, including Thr280, a novel, regulated site on a conserved residue near the TLT-1 ITIM regulatory sequence. SIGNIFICANCE: Platelet function relies on the secretion of active molecules from intracellular vesicles, or granules, which contain soluble and membrane-bound proteins that are essential for platelet aggregation, coagulation reactions, and pathogen defense mechanisms. TLT-1 is sequestered in α-granules and transported to the plasma membrane, where it plays a unique role in hemostasis after inflammatory insults. Despite the known importance of TLT-1 in platelet biology, our knowledge of TLT-1 mechanistic signaling is limited. This study defines the TLT-1 interactome in resting and active human platelets, identifying several novel TLT-1 interactors, as well as TLT-1 phosphorylation sites, all with likely signaling implications in platelet aggregation dynamics."xsd:string |
| http://purl.uniprot.org/citations/31923473 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jprot.2020.103638"xsd:string |
| http://purl.uniprot.org/citations/31923473 | http://purl.uniprot.org/core/author | "Ballif B.A."xsd:string |
| http://purl.uniprot.org/citations/31923473 | http://purl.uniprot.org/core/author | "Cruz C."xsd:string |
| http://purl.uniprot.org/citations/31923473 | http://purl.uniprot.org/core/author | "Washington A.V."xsd:string |
| http://purl.uniprot.org/citations/31923473 | http://purl.uniprot.org/core/author | "Fonseca K."xsd:string |
| http://purl.uniprot.org/citations/31923473 | http://purl.uniprot.org/core/author | "Schmoker A.M."xsd:string |
| http://purl.uniprot.org/citations/31923473 | http://purl.uniprot.org/core/author | "Melendez L.M."xsd:string |
| http://purl.uniprot.org/citations/31923473 | http://purl.uniprot.org/core/author | "Branfeild S."xsd:string |
| http://purl.uniprot.org/citations/31923473 | http://purl.uniprot.org/core/author | "Cantres Y.M."xsd:string |
| http://purl.uniprot.org/citations/31923473 | http://purl.uniprot.org/core/author | "Colon Flores L.G."xsd:string |
| http://purl.uniprot.org/citations/31923473 | http://purl.uniprot.org/core/author | "Pagan Torres F.D."xsd:string |
| http://purl.uniprot.org/citations/31923473 | http://purl.uniprot.org/core/author | "Perez Pearson L.M."xsd:string |
| http://purl.uniprot.org/citations/31923473 | http://purl.uniprot.org/core/author | "Salgado Ramirez C.A."xsd:string |
| http://purl.uniprot.org/citations/31923473 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
| http://purl.uniprot.org/citations/31923473 | http://purl.uniprot.org/core/name | "J Proteomics"xsd:string |
| http://purl.uniprot.org/citations/31923473 | http://purl.uniprot.org/core/pages | "103638"xsd:string |
| http://purl.uniprot.org/citations/31923473 | http://purl.uniprot.org/core/title | "Defining the TLT-1 interactome from resting and activated human platelets."xsd:string |
| http://purl.uniprot.org/citations/31923473 | http://purl.uniprot.org/core/volume | "215"xsd:string |
| http://purl.uniprot.org/citations/31923473 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/31923473 |
| http://purl.uniprot.org/citations/31923473 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/31923473 |
| http://purl.uniprot.org/uniprot/#_Q86YW5-mappedCitation-31923473 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31923473 |
| http://purl.uniprot.org/uniprot/Q86YW5 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/31923473 |