| http://purl.uniprot.org/citations/31933481 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31933481 | http://www.w3.org/2000/01/rdf-schema#comment | "Eukaryotic cell division requires the mitotic spindle, a microtubule (MT)-based structure which accurately aligns and segregates duplicated chromosomes. The dynamics of spindle formation are determined primarily by correctly localising the MT nucleator, γ-Tubulin Ring Complex (γ-TuRC), within the cell. A conserved MT-associated protein complex, Augmin, recruits γ-TuRC to pre-existing spindle MTs, amplifying their number, in an essential cellular phenomenon termed 'branching' MT nucleation. Here, we purify endogenous, GFP-tagged Augmin and γ-TuRC from Drosophila embryos to near homogeneity using a novel one-step affinity technique. We demonstrate that, in vitro, while Augmin alone does not affect Tubulin polymerisation dynamics, it stimulates γ-TuRC-dependent MT nucleation in a cell cycle-dependent manner. We also assemble and visualise the MT-Augmin-γ-TuRC-MT junction using light microscopy. Our work therefore conclusively reconstitutes branching MT nucleation. It also provides a powerful synthetic approach with which to investigate the emergence of cellular phenomena, such as mitotic spindle formation, from component parts."xsd:string |
| http://purl.uniprot.org/citations/31933481 | http://purl.org/dc/terms/identifier | "doi:10.7554/elife.49769"xsd:string |
| http://purl.uniprot.org/citations/31933481 | http://purl.uniprot.org/core/author | "Green L."xsd:string |
| http://purl.uniprot.org/citations/31933481 | http://purl.uniprot.org/core/author | "Tariq A."xsd:string |
| http://purl.uniprot.org/citations/31933481 | http://purl.uniprot.org/core/author | "Wakefield J.G."xsd:string |
| http://purl.uniprot.org/citations/31933481 | http://purl.uniprot.org/core/author | "Soeller C."xsd:string |
| http://purl.uniprot.org/citations/31933481 | http://purl.uniprot.org/core/author | "Jeynes J.C.G."xsd:string |
| http://purl.uniprot.org/citations/31933481 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
| http://purl.uniprot.org/citations/31933481 | http://purl.uniprot.org/core/name | "Elife"xsd:string |
| http://purl.uniprot.org/citations/31933481 | http://purl.uniprot.org/core/pages | "e49769"xsd:string |
| http://purl.uniprot.org/citations/31933481 | http://purl.uniprot.org/core/title | "In vitro reconstitution of branching microtubule nucleation."xsd:string |
| http://purl.uniprot.org/citations/31933481 | http://purl.uniprot.org/core/volume | "9"xsd:string |
| http://purl.uniprot.org/citations/31933481 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/31933481 |
| http://purl.uniprot.org/citations/31933481 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/31933481 |
| http://purl.uniprot.org/uniprot/#_E1JJQ3-mappedCitation-31933481 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31933481 |
| http://purl.uniprot.org/uniprot/#_E1JJQ4-mappedCitation-31933481 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31933481 |
| http://purl.uniprot.org/uniprot/#_Q7K4B4-mappedCitation-31933481 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31933481 |
| http://purl.uniprot.org/uniprot/#_Q86LC4-mappedCitation-31933481 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31933481 |
| http://purl.uniprot.org/uniprot/#_M9PDN9-mappedCitation-31933481 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31933481 |
| http://purl.uniprot.org/uniprot/#_P42271-mappedCitation-31933481 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31933481 |
| http://purl.uniprot.org/uniprot/#_Q8IQW7-mappedCitation-31933481 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31933481 |
| http://purl.uniprot.org/uniprot/#_Q9VAP2-mappedCitation-31933481 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31933481 |
| http://purl.uniprot.org/uniprot/#_Q8MT65-mappedCitation-31933481 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31933481 |
| http://purl.uniprot.org/uniprot/#_Q8SZD0-mappedCitation-31933481 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/31933481 |