| http://purl.uniprot.org/citations/31978092 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/31978092 | http://www.w3.org/2000/01/rdf-schema#comment | "Abnormal accumulation of acyl-CoA cholesterol acyltransferase-1 (ACAT-1) mediated cholesterol ester has been shown to contribute to cancer progression in various cancers including leukemia, glioma, breast, pancreatic and prostate cancers. However, the significance of ACAT-1 and cholesterol esters (CE) is relatively understudied in ovarian cancer. In this in vitro study, we assessed the expression and contribution of ACAT-1 in ovarian cancer progression. We observed a significant increase in the expression of ACAT-1 and CE levels in a panel of ovarian cancer cell lines (OC-314, SKOV-3 and IGROV-1) compared to primary ovarian epithelial cells (normal controls). To confirm the tumor promoting capacity of ACAT-1, we inhibited ACAT-1 expression and activity by treating our cell lines with an ACAT inhibitor, avasimibe, or by stable transfection with ACAT-1 specific short hairpin RNA (shRNA). We observed significant suppression of cell proliferation, migration and invasion in ACAT-1 knockdown ovarian cancer cell lines compared to their respective controls (cell lines transfected with scrambled shRNA). ACAT-1 inhibition enhanced apoptosis with a concurrent increase in caspases 3/7 activity and decreased mitochondrial membrane potential. Increased generation of reactive oxygen species (ROS) coupled with increased expression of p53 may be the mechanism(s) underlying pro-apoptotic action of ACAT-1 inhibition. Additionally, ACAT-1 inhibited ovarian cancer cell lines displayed enhanced chemosensitivity to cisplatin treatment. These results suggest ACAT-1 may be a potential new target for the treatment of ovarian cancer."xsd:string |
| http://purl.uniprot.org/citations/31978092 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0228024"xsd:string |
| http://purl.uniprot.org/citations/31978092 | http://purl.uniprot.org/core/author | "Wang X."xsd:string |
| http://purl.uniprot.org/citations/31978092 | http://purl.uniprot.org/core/author | "Brard L."xsd:string |
| http://purl.uniprot.org/citations/31978092 | http://purl.uniprot.org/core/author | "Ayyagari V.N."xsd:string |
| http://purl.uniprot.org/citations/31978092 | http://purl.uniprot.org/core/author | "Diaz-Sylvester P.L."xsd:string |
| http://purl.uniprot.org/citations/31978092 | http://purl.uniprot.org/core/author | "Groesch K."xsd:string |
| http://purl.uniprot.org/citations/31978092 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
| http://purl.uniprot.org/citations/31978092 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
| http://purl.uniprot.org/citations/31978092 | http://purl.uniprot.org/core/pages | "e0228024"xsd:string |
| http://purl.uniprot.org/citations/31978092 | http://purl.uniprot.org/core/title | "Assessment of acyl-CoA cholesterol acyltransferase (ACAT-1) role in ovarian cancer progression-An in vitro study."xsd:string |
| http://purl.uniprot.org/citations/31978092 | http://purl.uniprot.org/core/volume | "15"xsd:string |
| http://purl.uniprot.org/citations/31978092 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/31978092 |
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