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Background

Growing evidence shows that dysregulation of miRNAs plays a significant role in papillary thyroid cancer (PTC) tumorigenesis and development. The abnormal expression of miR-384 has been acknowledged in the proliferation or metastasis of some cancers. However, the function and the underlying mechanism of miR-384 in PTC progression remain largely unknown.

Methods

Real-time PCR was conducted to detect miR-384 expression in 58 cases of PTC and their adjacent noncancerous tissues. MTT, soft agar assay Transwell assay, and wound-healing assay were carried out to explore the biological function of miR-384 in PTC cell lines of BCPAP and K1. Bioinformatics analysis, dual-luciferase reporter assay, western blot, and functional complementation analysis were conducted to explore the target gene of miR-384. Moreover, Spearman's correlation analysis was conducted to reveal the correlation between miR-384 and PRKACB mRNA in PTC.

Results

The expression of miR-384 decreased obviously in PTC, especially in the tumors with lymph node metastasis or larger tumor size. The ectopic upregulation of miR-384 significantly suppressed PTC progression, and the inhibition of miR-384 had the opposite effects. Moreover, PRKACB gene was confirmed as the target of miR-384.

Conclusion

The study suggests that miR-384 serves as a tumor suppressor in PTC progression by directly targeting the 3'-UTR of PRKACB gene."xsd:string
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http://purl.uniprot.org/citations/31998791http://purl.uniprot.org/core/author"Qian X."xsd:string
http://purl.uniprot.org/citations/31998791http://purl.uniprot.org/core/author"Wang Y."xsd:string
http://purl.uniprot.org/citations/31998791http://purl.uniprot.org/core/author"Zhang X."xsd:string
http://purl.uniprot.org/citations/31998791http://purl.uniprot.org/core/author"Zhou H."xsd:string
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http://purl.uniprot.org/citations/31998791http://purl.uniprot.org/core/title"MicroRNA-384 Inhibits the Progression of Papillary Thyroid Cancer by Targeting PRKACB."xsd:string
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