| http://purl.uniprot.org/citations/32007275 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/32007275 | http://www.w3.org/2000/01/rdf-schema#comment | "The cMyb trans-activation domain is one of the model systems to understand the folding and binding mechanisms in intrinsically disordered proteins. cMyb (291-315) TAD (cMyb TAD) upon interaction with KIX plays a crucial role in transcriptional regulation. However, nothing is known regarding its aggregation behaviour on change of buffer conditions or stressed environment. Notably, most of the disease-associated amyloid-forming proteins such as Aβ, Tau, α-synuclein, and amylin are natively unstructured. Nevertheless, to date, very fewer evidence on aggregation behaviours on TAD domains are available. Therefore, this is necessary to investigate the aggregation propensity of intrinsically disordered cMyb TAD domain in isolation. As an essential step in that direction, we have extensively studied the aggregation behaviour of cMyb TAD using the standard approaches for aggregation studies and systematically probed the amyloid conformations. These aggregates are ThT and ANS-positive whose amyloid nature was also confirmed by Far-UV CD spectroscopic studies suggesting that cMyb TAD fibrils are rich in β-sheet secondary structure, transmission electron microscopy revealed the formation of characteristic long branched amyloid fibrils of 6-16 nm diameter, and MTT assay in SH-SY5Y neuroblastoma cells suggest that these aggregates are cytotoxic. This amyloid nature of cMyb TAD may affect its binding with KIX and alter cMyb function (transcriptional regulation) under acidic/stressed conditions."xsd:string |
| http://purl.uniprot.org/citations/32007275 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2020.01.110"xsd:string |
| http://purl.uniprot.org/citations/32007275 | http://purl.uniprot.org/core/author | "Giri R."xsd:string |
| http://purl.uniprot.org/citations/32007275 | http://purl.uniprot.org/core/author | "Gadhave K."xsd:string |
| http://purl.uniprot.org/citations/32007275 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
| http://purl.uniprot.org/citations/32007275 | http://purl.uniprot.org/core/name | "Biochem Biophys Res Commun"xsd:string |
| http://purl.uniprot.org/citations/32007275 | http://purl.uniprot.org/core/pages | "446-452"xsd:string |
| http://purl.uniprot.org/citations/32007275 | http://purl.uniprot.org/core/title | "Amyloid formation by intrinsically disordered trans-activation domain of cMyb."xsd:string |
| http://purl.uniprot.org/citations/32007275 | http://purl.uniprot.org/core/volume | "524"xsd:string |
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