| http://purl.uniprot.org/citations/32015554 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/32015554 | http://www.w3.org/2000/01/rdf-schema#comment | "Neddylation is the post-translational protein modification most closely related to ubiquitination. Whereas the ubiquitin-like protein NEDD8 is well studied for its role in activating cullin-RING E3 ubiquitin ligases, little is known about other substrates. We developed serial NEDD8-ubiquitin substrate profiling (sNUSP), a method that employs NEDD8 R74K knock-in HEK293 cells, allowing discrimination of endogenous NEDD8- and ubiquitin-modification sites by MS after Lys-C digestion and K-εGG-peptide enrichment. Using sNUSP, we identified 607 neddylation sites dynamically regulated by the neddylation inhibitor MLN4924 and the de-neddylating enzyme NEDP1, implying that many non-cullin proteins are neddylated. Among the candidates, we characterized lysine 112 of the actin regulator cofilin as a novel neddylation event. Global inhibition of neddylation in developing neurons leads to cytoskeletal defects, altered actin dynamics and neurite growth impairments, whereas site-specific neddylation of cofilin at K112 regulates neurite outgrowth, suggesting that cofilin neddylation contributes to the regulation of neuronal actin organization."xsd:string |
| http://purl.uniprot.org/citations/32015554 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41594-019-0370-3"xsd:string |
| http://purl.uniprot.org/citations/32015554 | http://purl.uniprot.org/core/author | "Hoogenraad C.C."xsd:string |
| http://purl.uniprot.org/citations/32015554 | http://purl.uniprot.org/core/author | "Adrian M."xsd:string |
| http://purl.uniprot.org/citations/32015554 | http://purl.uniprot.org/core/author | "Sheng M."xsd:string |
| http://purl.uniprot.org/citations/32015554 | http://purl.uniprot.org/core/author | "Wurst W."xsd:string |
| http://purl.uniprot.org/citations/32015554 | http://purl.uniprot.org/core/author | "Kirkpatrick D.S."xsd:string |
| http://purl.uniprot.org/citations/32015554 | http://purl.uniprot.org/core/author | "Phu L."xsd:string |
| http://purl.uniprot.org/citations/32015554 | http://purl.uniprot.org/core/author | "Heidersbach A."xsd:string |
| http://purl.uniprot.org/citations/32015554 | http://purl.uniprot.org/core/author | "Verschueren E."xsd:string |
| http://purl.uniprot.org/citations/32015554 | http://purl.uniprot.org/core/author | "Refojo D."xsd:string |
| http://purl.uniprot.org/citations/32015554 | http://purl.uniprot.org/core/author | "Yankilevich P."xsd:string |
| http://purl.uniprot.org/citations/32015554 | http://purl.uniprot.org/core/author | "Becerra R."xsd:string |
| http://purl.uniprot.org/citations/32015554 | http://purl.uniprot.org/core/author | "Vogl A.M."xsd:string |
| http://purl.uniprot.org/citations/32015554 | http://purl.uniprot.org/core/author | "Giusti S.A."xsd:string |
| http://purl.uniprot.org/citations/32015554 | http://purl.uniprot.org/core/author | "Bordenave M.D."xsd:string |
| http://purl.uniprot.org/citations/32015554 | http://purl.uniprot.org/core/author | "Hinkle T.B."xsd:string |
| http://purl.uniprot.org/citations/32015554 | http://purl.uniprot.org/core/author | "Stefani F.D."xsd:string |
| http://purl.uniprot.org/citations/32015554 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
| http://purl.uniprot.org/citations/32015554 | http://purl.uniprot.org/core/name | "Nat Struct Mol Biol"xsd:string |
| http://purl.uniprot.org/citations/32015554 | http://purl.uniprot.org/core/pages | "210-220"xsd:string |
| http://purl.uniprot.org/citations/32015554 | http://purl.uniprot.org/core/title | "Global site-specific neddylation profiling reveals that NEDDylated cofilin regulates actin dynamics."xsd:string |
| http://purl.uniprot.org/citations/32015554 | http://purl.uniprot.org/core/volume | "27"xsd:string |
| http://purl.uniprot.org/citations/32015554 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/32015554 |