| http://purl.uniprot.org/citations/32041253 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/32041253 | http://www.w3.org/2000/01/rdf-schema#comment | "Transforming Growth Factor β (TGF-β) is involved in fibrosis as well as the regulation of muscle mass, and contributes to the progressive pathology of muscle wasting disorders. However, little is known regarding the time-dependent signalling of TGF-β in myoblasts and myotubes, as well as how TGF-β affects collagen type I expression and the phenotypes of these cells. Here, we assessed effects of TGF-β on gene expression in C2C12 myoblasts and myotubes after 1, 3, 9, 24 and 48 h treatment. In myoblasts, various myogenic genes were repressed after 9, 24 and 48 h, while in myotubes only a reduction in Myh3 expression was observed. In both myoblasts and myotubes, TGF-β acutely induced the expression of a subset of genes involved in fibrosis, such as Ctgf and Fgf-2, which was subsequently followed by increased expression of Col1a1. Knockdown of Ctgf and Fgf-2 resulted in a lower Col1a1 expression level. Furthermore, the effects of TGF-β on myogenic and fibrotic gene expression were more pronounced than those of myostatin, and knockdown of TGF-β type I receptor Tgfbr1, but not receptor Acvr1b, resulted in a reduction in Ctgf and Col1a1 expression. These results indicate that, during muscle regeneration, TGF-β induces fibrosis via Tgfbr1 by stimulating the autocrine signalling of Ctgf and Fgf-2."xsd:string |
| http://purl.uniprot.org/citations/32041253 | http://purl.org/dc/terms/identifier | "doi:10.3390/cells9020375"xsd:string |
| http://purl.uniprot.org/citations/32041253 | http://purl.uniprot.org/core/author | "Jaspers R.T."xsd:string |
| http://purl.uniprot.org/citations/32041253 | http://purl.uniprot.org/core/author | "Offringa C."xsd:string |
| http://purl.uniprot.org/citations/32041253 | http://purl.uniprot.org/core/author | "de Wit G.M.J."xsd:string |
| http://purl.uniprot.org/citations/32041253 | http://purl.uniprot.org/core/author | "Galli Caro R.A."xsd:string |
| http://purl.uniprot.org/citations/32041253 | http://purl.uniprot.org/core/author | "Hillege M.M.G."xsd:string |
| http://purl.uniprot.org/citations/32041253 | http://purl.uniprot.org/core/author | "Hoogaars W.M.H."xsd:string |
| http://purl.uniprot.org/citations/32041253 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
| http://purl.uniprot.org/citations/32041253 | http://purl.uniprot.org/core/name | "Cells"xsd:string |
| http://purl.uniprot.org/citations/32041253 | http://purl.uniprot.org/core/pages | "E375"xsd:string |
| http://purl.uniprot.org/citations/32041253 | http://purl.uniprot.org/core/title | "TGF-beta Regulates Collagen Type I Expression in Myoblasts and Myotubes via Transient pii>Ctgfpi/i> and pii>Fgf-2pi/i> Expression."xsd:string |
| http://purl.uniprot.org/citations/32041253 | http://purl.uniprot.org/core/volume | "9"xsd:string |
| http://purl.uniprot.org/citations/32041253 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/32041253 |
| http://purl.uniprot.org/citations/32041253 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/32041253 |
| http://purl.uniprot.org/uniprot/#_P11087-mappedCitation-32041253 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32041253 |
| http://purl.uniprot.org/uniprot/#_P15655-mappedCitation-32041253 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32041253 |
| http://purl.uniprot.org/uniprot/#_Q64353-mappedCitation-32041253 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32041253 |
| http://purl.uniprot.org/uniprot/#_P29268-mappedCitation-32041253 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32041253 |
| http://purl.uniprot.org/uniprot/#_Q925A1-mappedCitation-32041253 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32041253 |
| http://purl.uniprot.org/uniprot/#_Q925A2-mappedCitation-32041253 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32041253 |
| http://purl.uniprot.org/uniprot/#_Q925A3-mappedCitation-32041253 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32041253 |
| http://purl.uniprot.org/uniprot/#_Q99LL6-mappedCitation-32041253 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32041253 |
| http://purl.uniprot.org/uniprot/#_Q60785-mappedCitation-32041253 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32041253 |