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http://purl.uniprot.org/citations/32058015http://www.w3.org/2000/01/rdf-schema#comment"

Background

KCNE1 loss-of-function variants cause type 5 long QT syndrome (LQT5). However, most alleged LQT5-causative KCNE1 variants were identified before the true rate of background genetic variation was appreciated fully.

Objective

The purpose of this study was to reassess the clinical and electrophysiological (EP) phenotypes associated with KCNE1 variants detected in a single-center LQTS cohort.

Methods

Retrospective analysis of 1026 LQTS patients was used to identify those individuals with isolated KCNE1 ultra-rare variants (minor allele frequency [MAF] <0.0004 in the Genome Aggregation Database [gnomAD]). After classification according to American College of Medical Genetics (ACMG) guidelines, variants of uncertain significance (VUS) were characterized in vitro using whole-cell patch-clamp technique. Lastly, the clinical phenotype observed in ACMG pathogenic/likely pathogenic (P/LP) KCNE1-positive individuals was assessed.

Results

Overall, 6 KCNE1 variants were identified in 38 of 1026 LQTS patients (3.7%). Based on existing data, 2 KCNE1 variants (p.Asp76Asn-KCNE1 and p.Arg98Trp-KCNE1) were classified as P/LP. Whereas the p.Ser28Leu-KCNE1 VUS conferred a loss-of-function EP phenotype (72% reduction in IKs current) and was upgraded to an LP variant, the 3 remaining KCNE1 VUS (p.Arg67Cys-KCNE1, p.Arg67His-KCNE1, p.Ser74Leu-KCNE1) were indistinguishable from wild type. Collectively, the phenotype observed in p.Ser28Leu-KCNE1-, p.Asp76Asn-KCNE1-, and p.Arg98Trp-KCNE1-positive individuals (n = 22) was relatively weak (91% asymptomatic; average QTc 444 ± 19 ms; 27% with a maladaptive QTc response during exercise/recovery).

Conclusion

This study indicates that p.Ser28Leu-KCNE1 may be an LQT5-causative substrate analogous to p.Asp76Asn-KCNE1 and p.Arg98Trp-KCNE1. However, the weak phenotype and cumulative gnomAD MAF (42/141,156) associated with these P/LP variants suggest LQT5/KCNE-LQTS may be a more common/weaker form of LQTS than anticipated previously."xsd:string
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http://purl.uniprot.org/citations/32058015http://purl.uniprot.org/core/author"Ye D."xsd:string
http://purl.uniprot.org/citations/32058015http://purl.uniprot.org/core/author"Zhou W."xsd:string
http://purl.uniprot.org/citations/32058015http://purl.uniprot.org/core/author"Ackerman M.J."xsd:string
http://purl.uniprot.org/citations/32058015http://purl.uniprot.org/core/author"Tester D.J."xsd:string
http://purl.uniprot.org/citations/32058015http://purl.uniprot.org/core/author"Giudicessi J.R."xsd:string
http://purl.uniprot.org/citations/32058015http://purl.uniprot.org/core/author"Garmany R."xsd:string
http://purl.uniprot.org/citations/32058015http://purl.uniprot.org/core/date"2020"xsd:gYear
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http://purl.uniprot.org/citations/32058015http://purl.uniprot.org/core/title"Clinical and functional reappraisal of alleged type 5 long QT syndrome: Causative genetic variants in the KCNE1-encoded minK beta-subunit."xsd:string
http://purl.uniprot.org/citations/32058015http://purl.uniprot.org/core/volume"17"xsd:string
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