| http://purl.uniprot.org/citations/32060138 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/32060138 | http://www.w3.org/2000/01/rdf-schema#comment | "Notch signaling is emerging as a critical regulator of T cell activation and function. However, there is no reliable cell surface indicator of Notch signaling across activated T cell subsets. In this study, we show that Notch signals induce upregulated expression of the Gcnt1 glycosyltransferase gene in T cells mediating graft-versus-host disease after allogeneic bone marrow transplantation in mice. To determine if Gcnt1-mediated O-glycosylation could be used as a Notch signaling reporter, we quantified the core-2 O-glycoform of CD43 in multiple T cell subsets during graft-versus-host disease. Pharmacological blockade of Delta-like Notch ligands abrogated core-2 O-glycosylation in a dose-dependent manner after allogeneic bone marrow transplantation, both in donor-derived CD4+ and CD8+ effector T cells and in Foxp3+ regulatory T cells. CD43 core-2 O-glycosylation depended on cell-intrinsic canonical Notch signals and identified CD4+ and CD8+ T cells with high cytokine-producing ability. Gcnt1-deficient T cells still drove lethal alloreactivity, showing that core-2 O-glycosylation predicted, but did not cause, Notch-dependent T cell pathogenicity. Using core-2 O-glycosylation as a marker of Notch signaling, we identified Ccl19-Cre+ fibroblastic stromal cells as critical sources of Delta-like ligands in graft-versus-host responses irrespective of conditioning intensity. Core-2 O-glycosylation also reported Notch signaling in CD8+ T cell responses to dendritic cell immunization, Listeria infection, and viral infection. Thus, we uncovered a role for Notch in controlling core-2 O-glycosylation and identified a cell surface marker to quantify Notch signals in multiple immunological contexts. Our findings will help refine our understanding of the regulation, cellular source, and timing of Notch signals in T cell immunity."xsd:string |
| http://purl.uniprot.org/citations/32060138 | http://purl.org/dc/terms/identifier | "doi:10.4049/jimmunol.1901194"xsd:string |
| http://purl.uniprot.org/citations/32060138 | http://purl.uniprot.org/core/author | "Chung J."xsd:string |
| http://purl.uniprot.org/citations/32060138 | http://purl.uniprot.org/core/author | "Maillard I."xsd:string |
| http://purl.uniprot.org/citations/32060138 | http://purl.uniprot.org/core/author | "Ludewig B."xsd:string |
| http://purl.uniprot.org/citations/32060138 | http://purl.uniprot.org/core/author | "Koch U."xsd:string |
| http://purl.uniprot.org/citations/32060138 | http://purl.uniprot.org/core/author | "Radtke F."xsd:string |
| http://purl.uniprot.org/citations/32060138 | http://purl.uniprot.org/core/author | "Blazar B.R."xsd:string |
| http://purl.uniprot.org/citations/32060138 | http://purl.uniprot.org/core/author | "Carrington L."xsd:string |
| http://purl.uniprot.org/citations/32060138 | http://purl.uniprot.org/core/author | "Siebel C.W."xsd:string |
| http://purl.uniprot.org/citations/32060138 | http://purl.uniprot.org/core/author | "Labrecque N."xsd:string |
| http://purl.uniprot.org/citations/32060138 | http://purl.uniprot.org/core/author | "Brennan T.V."xsd:string |
| http://purl.uniprot.org/citations/32060138 | http://purl.uniprot.org/core/author | "Perkey E."xsd:string |
| http://purl.uniprot.org/citations/32060138 | http://purl.uniprot.org/core/author | "Nolz J."xsd:string |
| http://purl.uniprot.org/citations/32060138 | http://purl.uniprot.org/core/author | "Granadier D."xsd:string |
| http://purl.uniprot.org/citations/32060138 | http://purl.uniprot.org/core/author | "Dils A."xsd:string |
| http://purl.uniprot.org/citations/32060138 | http://purl.uniprot.org/core/author | "Maurice De Sousa D."xsd:string |
| http://purl.uniprot.org/citations/32060138 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
| http://purl.uniprot.org/citations/32060138 | http://purl.uniprot.org/core/name | "J Immunol"xsd:string |
| http://purl.uniprot.org/citations/32060138 | http://purl.uniprot.org/core/pages | "1674-1688"xsd:string |
| http://purl.uniprot.org/citations/32060138 | http://purl.uniprot.org/core/title | "GCNT1-Mediated O-Glycosylation of the Sialomucin CD43 Is a Sensitive Indicator of Notch Signaling in Activated T Cells."xsd:string |
| http://purl.uniprot.org/citations/32060138 | http://purl.uniprot.org/core/volume | "204"xsd:string |
| http://purl.uniprot.org/citations/32060138 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/32060138 |
| http://purl.uniprot.org/citations/32060138 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/32060138 |