| http://purl.uniprot.org/citations/32079655 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/32079655 | http://www.w3.org/2000/01/rdf-schema#comment | "F-actin dynamics are known to control insulin secretion, but the point of intersection with the stimulus-secretion cascade is unknown. Here, using multiphoton imaging of β cells isolated from Lifeact-GFP transgenic mice, we show that glucose stimulation does not cause global changes in subcortical F-actin. Instead, we observe spatially discrete and transient F-actin changes around each fusing granule. This F-actin remodelling is dependent on actin nucleation and is observed for granule fusion induced by either glucose or high potassium stimulation. Using GFP-labelled proteins, we identify local enrichment of Arp3, dynamin 2 and clathrin, all occurring after granule fusion, suggesting early recruitment of an endocytic complex to the fusing granules. Block of Arp2/3 activity with drugs or shRNA inhibits F-actin coating, traps granules at the cell membrane and reduces insulin secretion. Block of formin-mediated actin nucleation also blocks F-actin coating, but has no effect on insulin secretion. We conclude that local Arp2/3-dependent actin nucleation at the sites of granule fusion plays an important role in post-fusion granule dynamics and in the regulation of insulin secretion."xsd:string |
| http://purl.uniprot.org/citations/32079655 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.236794"xsd:string |
| http://purl.uniprot.org/citations/32079655 | http://purl.uniprot.org/core/author | "Chang J."xsd:string |
| http://purl.uniprot.org/citations/32079655 | http://purl.uniprot.org/core/author | "Ma W."xsd:string |
| http://purl.uniprot.org/citations/32079655 | http://purl.uniprot.org/core/author | "Tong J."xsd:string |
| http://purl.uniprot.org/citations/32079655 | http://purl.uniprot.org/core/author | "Thorn P."xsd:string |
| http://purl.uniprot.org/citations/32079655 | http://purl.uniprot.org/core/author | "Kebede M.A."xsd:string |
| http://purl.uniprot.org/citations/32079655 | http://purl.uniprot.org/core/author | "Yau B."xsd:string |
| http://purl.uniprot.org/citations/32079655 | http://purl.uniprot.org/core/author | "Ho U."xsd:string |
| http://purl.uniprot.org/citations/32079655 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
| http://purl.uniprot.org/citations/32079655 | http://purl.uniprot.org/core/name | "J Cell Sci"xsd:string |
| http://purl.uniprot.org/citations/32079655 | http://purl.uniprot.org/core/pages | "jcs236794"xsd:string |
| http://purl.uniprot.org/citations/32079655 | http://purl.uniprot.org/core/title | "Arp2/3 nucleates F-actin coating of fusing insulin granules in pancreatic beta cells to control insulin secretion."xsd:string |
| http://purl.uniprot.org/citations/32079655 | http://purl.uniprot.org/core/volume | "133"xsd:string |
| http://purl.uniprot.org/citations/32079655 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/32079655 |
| http://purl.uniprot.org/citations/32079655 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/32079655 |
| http://purl.uniprot.org/uniprot/#_A0A087WPR6-mappedCitation-32079655 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32079655 |
| http://purl.uniprot.org/uniprot/#_A0A087WQ14-mappedCitation-32079655 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32079655 |
| http://purl.uniprot.org/uniprot/#_A0A087WQ83-mappedCitation-32079655 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32079655 |
| http://purl.uniprot.org/uniprot/#_A0A087WP86-mappedCitation-32079655 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32079655 |
| http://purl.uniprot.org/uniprot/#_A0A087WS98-mappedCitation-32079655 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32079655 |
| http://purl.uniprot.org/uniprot/#_A0A087WRA1-mappedCitation-32079655 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32079655 |
| http://purl.uniprot.org/uniprot/#_Q3ULF7-mappedCitation-32079655 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32079655 |
| http://purl.uniprot.org/uniprot/#_Q3UGH0-mappedCitation-32079655 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32079655 |