| http://purl.uniprot.org/citations/32139698 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/32139698 | http://www.w3.org/2000/01/rdf-schema#comment | "Transcription of the ribosomal RNA precursor by RNA polymerase (Pol) I is a prerequisite for the biosynthesis of ribosomes in eukaryotes. Compared to Pols II and III, the mechanisms underlying promoter recognition, initiation complex formation and DNA melting by Pol I substantially diverge. Here, we report the high-resolution cryo-EM reconstruction of a Pol I early initiation intermediate assembled on a double-stranded promoter scaffold that prevents the establishment of downstream DNA contacts. Our analyses demonstrate how efficient promoter-backbone interaction is achieved by combined re-arrangements of flexible regions in the 'core factor' subunits Rrn7 and Rrn11. Furthermore, structure-function analysis illustrates how destabilization of the melted DNA region correlates with contraction of the polymerase cleft upon transcription activation, thereby combining promoter recruitment with DNA-melting. This suggests that molecular mechanisms and structural features of Pol I initiation have co-evolved to support the efficient melting, initial transcription and promoter clearance required for high-level rRNA synthesis."xsd:string |
| http://purl.uniprot.org/citations/32139698 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41467-020-15052-y"xsd:string |
| http://purl.uniprot.org/citations/32139698 | http://purl.uniprot.org/core/author | "Engel C."xsd:string |
| http://purl.uniprot.org/citations/32139698 | http://purl.uniprot.org/core/author | "Pilsl M."xsd:string |
| http://purl.uniprot.org/citations/32139698 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
| http://purl.uniprot.org/citations/32139698 | http://purl.uniprot.org/core/name | "Nat Commun"xsd:string |
| http://purl.uniprot.org/citations/32139698 | http://purl.uniprot.org/core/pages | "1206"xsd:string |
| http://purl.uniprot.org/citations/32139698 | http://purl.uniprot.org/core/title | "Structural basis of RNA polymerase I pre-initiation complex formation and promoter melting."xsd:string |
| http://purl.uniprot.org/citations/32139698 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/32139698 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/32139698 |
| http://purl.uniprot.org/citations/32139698 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/32139698 |
| http://purl.uniprot.org/uniprot/#_P50106-mappedCitation-32139698 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32139698 |
| http://purl.uniprot.org/uniprot/#_P28000-mappedCitation-32139698 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32139698 |
| http://purl.uniprot.org/uniprot/#_P07703-mappedCitation-32139698 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32139698 |
| http://purl.uniprot.org/uniprot/#_P40992-mappedCitation-32139698 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32139698 |
| http://purl.uniprot.org/uniprot/#_P46669-mappedCitation-32139698 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32139698 |
| http://purl.uniprot.org/uniprot/#_P47006-mappedCitation-32139698 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32139698 |
| http://purl.uniprot.org/uniprot/#_P10964-mappedCitation-32139698 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32139698 |
| http://purl.uniprot.org/uniprot/#_Q04712-mappedCitation-32139698 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32139698 |
| http://purl.uniprot.org/uniprot/#_P22138-mappedCitation-32139698 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32139698 |
| http://purl.uniprot.org/uniprot/#_P22139-mappedCitation-32139698 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32139698 |
| http://purl.uniprot.org/uniprot/#_P40422-mappedCitation-32139698 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32139698 |
| http://purl.uniprot.org/uniprot/#_Q01080-mappedCitation-32139698 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32139698 |
| http://purl.uniprot.org/uniprot/#_P20434-mappedCitation-32139698 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32139698 |