| http://purl.uniprot.org/citations/32156700 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/32156700 | http://www.w3.org/2000/01/rdf-schema#comment | "Sperm head shaping is a key event in spermiogenesis and is tightly controlled via the acrosome-manchette network. Linker of nucleoskeleton and cytoskeleton (LINC) complexes consist of Sad1 and UNC84 domain-containing (SUN) and Klarsicht/ANC-1/Syne-1 homology (KASH) domain proteins and form conserved nuclear envelope bridges implicated in transducing mechanical forces from the manchette to sculpt sperm nuclei into a hook-like shape. However, the role of LINC complexes in sperm head shaping is still poorly understood. Here we assessed the role of SUN3, a testis-specific LINC component harboring a conserved SUN domain, in spermiogenesis. We show that CRISPR/Cas9-generated Sun3 knockout male mice are infertile, displaying drastically reduced sperm counts and a globozoospermia-like phenotype, including a missing, mislocalized, or fragmented acrosome, as well as multiple defects in sperm flagella. Further examination revealed that the sperm head abnormalities are apparent at step 9 and that the sperm nuclei fail to elongate because of the absence of manchette microtubules and perinuclear rings. These observations indicate that Sun3 deletion likely impairs the ability of the LINC complex to transduce the cytoskeletal force to the nuclear envelope, required for sperm head elongation. We also found that SUN3 interacts with SUN4 in mouse testes and that the level of SUN4 proteins is drastically reduced in Sun3-null mice. Altogether, our results indicate that SUN3 is essential for sperm head shaping and male fertility, providing molecular clues regarding the underlying pathology of the globozoospermia-like phenotype."xsd:string |
| http://purl.uniprot.org/citations/32156700 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.ra119.012375"xsd:string |
| http://purl.uniprot.org/citations/32156700 | http://purl.uniprot.org/core/author | "Jiang X."xsd:string |
| http://purl.uniprot.org/citations/32156700 | http://purl.uniprot.org/core/author | "Ma H."xsd:string |
| http://purl.uniprot.org/citations/32156700 | http://purl.uniprot.org/core/author | "Yu C."xsd:string |
| http://purl.uniprot.org/citations/32156700 | http://purl.uniprot.org/core/author | "Shi Q."xsd:string |
| http://purl.uniprot.org/citations/32156700 | http://purl.uniprot.org/core/author | "Gao Q."xsd:string |
| http://purl.uniprot.org/citations/32156700 | http://purl.uniprot.org/core/author | "Khan R."xsd:string |
| http://purl.uniprot.org/citations/32156700 | http://purl.uniprot.org/core/author | "Alsheimer M."xsd:string |
| http://purl.uniprot.org/citations/32156700 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
| http://purl.uniprot.org/citations/32156700 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/32156700 | http://purl.uniprot.org/core/pages | "6289-6298"xsd:string |
| http://purl.uniprot.org/citations/32156700 | http://purl.uniprot.org/core/title | "The testis-specific LINC component SUN3 is essential for sperm head shaping during mouse spermiogenesis."xsd:string |
| http://purl.uniprot.org/citations/32156700 | http://purl.uniprot.org/core/volume | "295"xsd:string |
| http://purl.uniprot.org/citations/32156700 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/32156700 |
| http://purl.uniprot.org/citations/32156700 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/32156700 |
| http://purl.uniprot.org/uniprot/#_E9LV81-mappedCitation-32156700 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32156700 |
| http://purl.uniprot.org/uniprot/#_Q5SS91-mappedCitation-32156700 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32156700 |
| http://purl.uniprot.org/uniprot/E9LV81 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/32156700 |
| http://purl.uniprot.org/uniprot/Q5SS91 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/32156700 |