| http://purl.uniprot.org/citations/32173470 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/32173470 | http://www.w3.org/2000/01/rdf-schema#comment | "The sodium-coupled bicarbonate cotransporter 1 (NBCe1) plays an essential role in the maintenance of acid-base homeostasis in the human body. However, little research has been done regarding the modification of NBCe1. Nedd4-2 is one of the most important ubiquitin E3 ligases in the kidney where it is responsible for mediating the ubiquitylation level of many important ion channel proteins; therefore, influencing their expression and membrane localization. In this study, we performed experiments based on a prediction from bioinformatics analysis that NBCe1 might be a Nedd4-2 target protein. The results of co-immunoprecipitation and glutathione S-transferase pull-down assays showed that Nedd4-2 interacted with NBCe1. An in vitro ubiquitination assay further demonstrated that Nedd4-2 is indeed the NBCe1 ubiquitin E3 ligase. The overexpression of Nedd4-2 decreased NBCe1 expression, while MG132 rescued the changes. Nedd4-2 overexpression also altered the subcellular distribution of NBCe1. Furthermore, the kidney specific Nedd4-2-knockout mice certified the alteration of NBCe1. In addition, we speculate that neddylation activates Nedd4-2. A co-immunoprecipitation analysis indicated that Nedd4-2 interacted with Nedd8. In vitro neddylation experiments further demonstrated that Nedd4-2 underwent neddylation modification. The overexpression of Nedd8 led to decreased NBCe1 expression, while Nedd4-2 inhibition rescued the changes. These findings demonstrate that Nedd4-2 acts as the ubiquitin E3 ligase of NBCe1, mediating the degradation and altering the subcellular distribution of NBCe1, and that the neddylation modification downregulated NBCe1 expression by upregulating Nedd4-2 activity."xsd:string |
| http://purl.uniprot.org/citations/32173470 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.yexcr.2020.111958"xsd:string |
| http://purl.uniprot.org/citations/32173470 | http://purl.uniprot.org/core/author | "Chang W."xsd:string |
| http://purl.uniprot.org/citations/32173470 | http://purl.uniprot.org/core/author | "Fang G."xsd:string |
| http://purl.uniprot.org/citations/32173470 | http://purl.uniprot.org/core/author | "Zhang B."xsd:string |
| http://purl.uniprot.org/citations/32173470 | http://purl.uniprot.org/core/author | "Zhao Y."xsd:string |
| http://purl.uniprot.org/citations/32173470 | http://purl.uniprot.org/core/author | "Tu J."xsd:string |
| http://purl.uniprot.org/citations/32173470 | http://purl.uniprot.org/core/date | "2020"xsd:gYear |
| http://purl.uniprot.org/citations/32173470 | http://purl.uniprot.org/core/name | "Exp Cell Res"xsd:string |
| http://purl.uniprot.org/citations/32173470 | http://purl.uniprot.org/core/pages | "111958"xsd:string |
| http://purl.uniprot.org/citations/32173470 | http://purl.uniprot.org/core/title | "Neddylation-mediated Nedd4-2 activation regulates ubiquitination modification of renal NBCe1."xsd:string |
| http://purl.uniprot.org/citations/32173470 | http://purl.uniprot.org/core/volume | "390"xsd:string |
| http://purl.uniprot.org/citations/32173470 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/32173470 |
| http://purl.uniprot.org/citations/32173470 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/32173470 |
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| http://purl.uniprot.org/uniprot/#_D3Z7I7-mappedCitation-32173470 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/32173470 |
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